Evaluation of significant gene expression changes in congenital and acquired cholesteatoma

Etiopathogenesis of acquired and congenital cholesteatoma is still unclear. The clinical behavior of adult acquired, pediatric acquired and congenital cholesteatomas show differences. The scope of the this study was to detect the matrix metalloproteinase ( MMP ), tissue inhibitors of metalloproteinase ( TIMP ) and epidermal growth factor receptor ( EGFR ) gene expression changes in cholesteatoma perimatrix and to compare these changes among congenital cholesteatoma, adult acquired cholesteatoma and pediatric acquired cholesteatoma. A total of 16 genes including MMP s, TIMP s and EGFR were analyzed in the samples of 32 cholesteatoma tissues. Real-time PCR was used for detection of the gene expression levels. Data analyses were achieved by ΔΔCT method (Light Cycler 480 Quantification Software) and Statistical Package for Social Sciences (SPSS) version 22.0. The expression levels of MMP-2 , -9 , -10 , -11 , -13 , -14 , -15 , -16 and EGFR genes were significantly higher in acquired cholesteatoma than healthy tissue (p