Effect of pH on volatile fatty acid production from anaerobic digestion of potato peel waste

•The highest concentration and yield of VFAs were achieved with pH 7.0.•Butyric acid and acetic acid was the dominant product under acidic and alkaline pH, respectively.•When the NADH level achieves a relatively low state, VFA production is improved.•Alkaline and neutral pH had higher hydrolysis efficiency than acidic pH. In this study, potato peel waste was used as feedstock to produce volatile fatty acids (VFAs) by anaerobic digestion. The effects of different pH levels (pH 5.0, pH 7.0, pH 11.0, and uncontrolled pH) on VFA concentration and composition, intermediate products, and metabolic state were evaluated. The results showed that the highest total VFA production was achieved with pH 7.0 (41.9 g COD/L and 632.2 mg COD/g VSfed), followed by that with uncontrolled pH. Butyric acid was the dominant product under acidic pH, whereas acetic acid dominated under alkaline pH. The type of acidogenic fermentation at pH 7.0 was the mixed-acid type. The change in NADH level in the mixed-acid type of fermentation consisted of small fluctuations, enhancing the stability and efficiency of fermentation. The enzymatic activities of acetate kinase and butyrate kinase were slightly inhibited at pH 5.0 and 11.0, resulting in relatively low VFAs production.