Development of a Piezoelectric PVDF‐TrFE Fibrous Scaffold to Guide Cell Adhesion, Proliferation, and Alignment

Severe peripheral nervous system injuries currently hold limited therapeutic solutions. Existing clinical techniques such as autografts, allografts, and newer nerve guidance conduits have shown variable outcomes in functional recovery, adverse immune responses, and in some cases low or minimal availability. This can be attributed in part to the lack of chemical, physical, and electrical cues directing both nerve guidance and regeneration. To address this pressing clinical issue, electrospun nanofibers and microfibers composed of piezoelectric polyvinylidene flouride‐triflouroethylene (PVDF‐TrFE) have been introduced as an alternative template for tissue engineered biomaterials, specifically as it pertains to their relevance in soft tissue and nerve repair. Here, biocompatible scaffolds of PVDF‐TrFE are fabricated and their ability to generate an electrical response to mechanical deformations and produce a suitable regenerative microenvironment is examined. It is determined that 20% (w/v) PVDF‐TrFE in (6:4) dimethyl formamide (DMF):acetone solvent maintains a desirable piezoelectric coefficient and the proper physical and electrical characteristics for tissue regeneration. Further, it is concluded that scaffolds of varying thickness promoted the adhesion and alignment of Schwann cells and fibroblasts. This work offers a prelude to further advancements in nanofibrous technology and a promising outlook for alternative, autologous remedies to peripheral nerve damage. PVDF‐TrFE piezoelectric fibrous scaffolds are electrospun with tunable physical, chemical, and electrical properties and demonstrated as a biomaterial for tissue engineering. The physical, piezoelectric, and cell‐ECM characteristics of PVDF‐TrFE are determined for different electrospinning parameters. Moving forward, this biomaterial has potential to lead to novel therapies utilizing the material's intrinsic piezoelectricity as a source for electrode‐free stimulation in regenerative medicine.