Immunological profile of periapical endodontic infection in patients undergoing haematopoietic transplantation

Objectives To evaluate the mRNA expression levels of cytokines interferon-γ, tumour necrosis factor-α, interleukin IL-1β, IL-10, and the chemokine CCL2/MCP-1, CCL4, and CXCR4 in the periapical interstitial fluid from root canal infections before and after bacterial load reduction in patients undergo...

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Veröffentlicht in:Clinical oral investigations 2021-03, Vol.25 (3), p.1403-1410
Hauptverfasser: Braga Diniz, Julia Mourão, Espaladori, Marcela Carvalho, e Souza Silva, Maria Elisa, de Brito, Luciana Carla Neves, Vieira, Leda Quercia, Sobrinho, Antônio Paulino Ribeiro
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Sprache:eng
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Zusammenfassung:Objectives To evaluate the mRNA expression levels of cytokines interferon-γ, tumour necrosis factor-α, interleukin IL-1β, IL-10, and the chemokine CCL2/MCP-1, CCL4, and CXCR4 in the periapical interstitial fluid from root canal infections before and after bacterial load reduction in patients undergoing haematopoietic stem cell transplantation (HSCT). Materials and methods The case group was composed of 10 patients undergoing HSCT, and our control group included 10 healthy patients. Clinical samples were taken from teeth with pulp necrosis. Three paper points were placed in the RCS and maintained for 2 min for microbial evaluation before cleaning and shaping procedures. After cleaning and drying the canal, three paper points were introduced into the root canal, passing passively through the root apex (2 mm) into the periapical tissues for 1 min. Samples were collected immediately after root canal cleaning and 7 days later (restrained root canal bacterial load) to characterize gene expression using real-time PCR. Results The results showed significantly reduction in the microbial load on day 7. An increased expression level of TNF-α and IFN-γ on day 7 in control and case groups was observed ( p  
ISSN:1432-6981
1436-3771
DOI:10.1007/s00784-020-03448-5