Calcium hypochlorite on mouse embryonic fibroblast cells (NIH3T3) in vitro cytotoxicity and genotoxicity: MTT and comet assay

Antimicrobial irrigation solutions are widely used under clinical settings. Their effect on dental tissue is a subject of recent research, which aims for a safer irrigant for clinical use. In this regard, here our goal was to evaluate the cytotoxicity and the genotoxicity of calcium hypochlorite (Ca...

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Veröffentlicht in:	Molecular biology reports 2020-07, Vol.47 (7), p.5377-5383
Hauptverfasser:	Yilmaz, Şehnaz, Yoldas, Oguz, Dumani, Aysin, Guler, Gizem, Ilgaz, Seda, Akbal, Eylül, Oksuz, Hale, Celik, Ayla, Yilmaz, Bertan
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal Anatomy Animal Biochemistry Biomedical and Life Sciences Calcium hypochlorite Cell number Cell proliferation Cell viability Comet assay Cytotoxicity Embryo fibroblasts Fibroblasts Genotoxicity Histology Life Sciences Morphology Original Article Sodium hypochlorite
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Zusammenfassung:	Antimicrobial irrigation solutions are widely used under clinical settings. Their effect on dental tissue is a subject of recent research, which aims for a safer irrigant for clinical use. In this regard, here our goal was to evaluate the cytotoxicity and the genotoxicity of calcium hypochlorite (Ca(OCl) 2 ) solution, along with NaOCl, on Mouse embryonic fibroblast cells (NIH3T3). First, Cells were treated either with NaOCl or Ca(OCl) 2 in a time- and dose-dependent manner for cytotoxicity by 3-(4,5-dimethylthiazolyl-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, then cell viability was calculated according to cell proliferation plots. Secondly, genotoxicity was assessed by Comet assay. Data were statistically analyzed by Tukey’s test ( P
ISSN:	0301-4851 1573-4978
DOI:	10.1007/s11033-020-05624-y