A convenient and rapid method for detecting d-glucose in honey used smartphone
[Display omitted] •The d-glucose dehydrogenase with His-tag was expressed in E. coli.•The color reaction system the enzyme catalyzed for detection of d-glucose was constructed.•Smart phone and Microsoft Photoshop was used for analyzing color.•The detection limit of the biosensor was 25 mM to 200 mM...
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Veröffentlicht in: | Food chemistry 2020-11, Vol.331, p.127348-127348, Article 127348 |
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Sprache: | eng |
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•The d-glucose dehydrogenase with His-tag was expressed in E. coli.•The color reaction system the enzyme catalyzed for detection of d-glucose was constructed.•Smart phone and Microsoft Photoshop was used for analyzing color.•The detection limit of the biosensor was 25 mM to 200 mM of d-glucose.
Information concerning food composition, including information on its glucose content, is essential for modern food industry due to greater consumer awareness and expectations. In this work, the gene encoding d-glucose dehydrogenase (GDH) from Bacillus Natto was expressed in Escherichia coli BL21(DE3) firstly. Ni-IDA column was used for the purification of GDH. Then, the purified GDH was used to construct a color system with stable and effective measurement of concentration of d-glucose. The smart phone photographing and the software Microsoft Photoshop have been used in the system for determination of the color. The enzymatic analysis system can detect the concentration of d-glucose from 5 mM to 40 mM, and other various sugars has no interference to the system. The system was used to quantitatively detect the concentration of d-glucose in honey. The system can be used for convenient and rapid detection of d-glucose in food, especially for large numbers of samples. |
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ISSN: | 0308-8146 1873-7072 |
DOI: | 10.1016/j.foodchem.2020.127348 |