Effects of sex and age on parental motivation in adult virgin California mice

•We compared parental motivation in male and female adult virgin California mice.•Males were more parentally motivated than females in two of three behavioral tests.•Parental motivation did not differ markedly between middle-aged and older adults.•T-maze tests provide complementary information to standard parental-behavior tests. Female mammals often demonstrate a rapid initiation of maternal responsiveness immediately after giving birth, as a result of neuroendocrine changes that occur during pregnancy and parturition. However, fathers and virgins of some species may display infant care similar to that performed by mothers but without experiencing these physiological events. In biparental species, in which both mothers and fathers care for their offspring, both sex and age may affect parental motivation, even in adult virgins. We examined the effects of sex and age on parental motivation in the California mouse, a monogamous, biparental rodent. We compared parental motivation of male and female virgins in both mid- and old adulthood using two new tests – a T-maze test and a rain test – as well as in standard parental-behavior tests. Adult virgin males were more parentally motivated than adult virgin females in both the T-maze test and the parental-behavior test, but parental motivation did not differ markedly between middle-aged and older adults of either sex. These findings suggest that sex differences in parental motivation in adult virgins are similar to those observed in other biparental rodents, and indicate that the T-maze test may be useful for evaluating parental motivation in this species.