Canditate metabolites for ash dieback tolerance in Fraxinus excelsior

In Fraxinus excelsior we dentified two chemotypes and 64 metabolites associated with increased or reduced susceptibility to ash dieback. Fraxetin and esculetin were validated as markers for tolerance to ash dieback. Abstract Ash dieback, a forest epidemic caused by the invasive fungus Hymenoscyphus...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of experimental botany 2020-10, Vol.71 (19), p.6074-6083
Hauptverfasser: Nemesio-Gorriz, Miguel, Menezes, Riya C, Paetz, Christian, Hammerbacher, Almuth, Steenackers, Marijke, Schamp, Kurt, Höfte, Monica, Svatoš, Aleš, Gershenzon, Jonathan, Douglas, Gerry C
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:In Fraxinus excelsior we dentified two chemotypes and 64 metabolites associated with increased or reduced susceptibility to ash dieback. Fraxetin and esculetin were validated as markers for tolerance to ash dieback. Abstract Ash dieback, a forest epidemic caused by the invasive fungus Hymenoscyphus fraxineus, threatens ash trees throughout Europe. Within Fraxinus excelsior populations, a small proportion of genotypes show a low susceptibility to the pathogen. We compared the metabolomes from a cohort of low-susceptibility ash genotypes with a cohort of high-susceptibility ash genotypes. This revealed two significantly different chemotypes. A total of 64 candidate metabolites associated with reduced or increased susceptibility in the chemical families secoiridoids, coumarins, flavonoids, phenylethanoids, and lignans. Increased levels of two coumarins, fraxetin and esculetin, were strongly associated with reduced susceptibility to ash dieback. Both coumarins inhibited the growth of H. fraxineus in vitro when supplied at physiological concentrations, thereby validating their role as markers for low susceptibility to ash dieback. Similarly, fungal growth inhibition was observed when the methanolic bark extract of low-susceptibility ash genotypes was supplied. Our findings indicate the presence of constitutive chemical defense barriers against ash dieback in ash.
ISSN:0022-0957
1460-2431
DOI:10.1093/jxb/eraa306