Improving glucose metabolism in the auditory cortex delays the aging of auditory function of guinea pig

•Glucose metabolism was firstly detected by micro PET/CT in auditory cortex of guinea pigs with ARHL.•The proteome were identified in auditory cortex of guinea pigs with ARHL by 2-DE and MALDI-TOF-MS.•Seven metabolism-related proteins may be an important role in the aging of auditory function in the guinea pigs by reducing glucose metabolism.•Improving glucose metabolism in the auditory cortex can prevent the aging of auditory function of guinea pig with ARHL induced by d-galactose. The glucose homeostasis is essential for brain function, and energy deficiency is a key feature of brain aging. We investigated whether improving glucose metabolism in the auditory cortex can delay the aging of auditory function of guinea pigs with age-related hearing loss (ARHL) by d-galactose. Auditory function was assessed by auditory brainstem response (ABR), glucose metabolism was detected by micro PET/CT, and the proteome were identified in auditory cortex by two-dimensional electrophoresis and matrix assisted laser desorption/ionization mass spectrometry. Glucose metabolism decreased in the auditory cortex of d-galactose group, and improving glucose metabolism can delay the aging of auditory function by upregulating seven metabolism-related proteins including ATP synthase subunit beta, triosephosphate isomerase, creatine kinase U-type, pyruvate dehydrogenase E1 component subunit beta, alpha-enolase, phosphoglycerate kinase, and tubulin beta-2A chain. These results suggest that the decrease of glucose metabolism in the auditory cortex may be an important role in the aging of auditory function, and improving glucose metabolism in the auditory cortex can delay the aging of auditory function of guinea pig with ARHL induced by d-galactose.