Thermoplastic microfluidic bioreactors with integrated electrodes to study tumor treating fields on yeast cells

Tumor-treating fields (TTFields) are alternating electrical fields of intermediate frequency and low intensity that can slow or inhibit tumor growth by disrupting mitosis division of cancerous cells through cell cycle proteins. In this work, for the first time, an in-house fabricated cyclo-olefin po...

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Veröffentlicht in:	Biomicrofluidics 2020-05, Vol.14 (3), p.034104-034104, Article 034104
Hauptverfasser:	Gencturk, Elif, Ulgen, Kutlu O., Mutlu, Senol
Format:	Artikel
Sprache:	eng
Schlagworte:	Biochemical Research Methods Biochemistry & Molecular Biology Biophysics Bioreactors Cell cycle Electrodes Electroporation Fluorescence Life Sciences & Biomedicine Microfluidics Mitosis Nanoscience & Nanotechnology Physical Sciences Physics Physics, Fluids & Plasmas Polyolefins Proteins Regular Science & Technology Science & Technology - Other Topics Tumors Yeast
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Zusammenfassung:	Tumor-treating fields (TTFields) are alternating electrical fields of intermediate frequency and low intensity that can slow or inhibit tumor growth by disrupting mitosis division of cancerous cells through cell cycle proteins. In this work, for the first time, an in-house fabricated cyclo-olefin polymer made microfluidic bioreactors are integrated with Cr/Au interdigitated electrodes to test TTFields on yeast cells with fluorescent protein:Nop56 gene. A small gap between electrodes (50 μm) allows small voltages (
ISSN:	1932-1058 1932-1058
DOI:	10.1063/5.0008462