Glaciimonas soli sp. nov., a soil bacterium isolated from the forest of a high elevation mountain

A Gram-negative, psychrophilic bacterium, designated strain GS1 T , was isolated from a forest soil sample collected from the West Peak of Mt. Yushan, Yushan National Park, Taiwan. Cells grown in broth cultures were mostly non-motile and non-flagellated, whereas motile cells with monotrichous, subpolar flagella were also observed. The novel strain grew over a temperature range of 4–25 °C with optimum growth at 10–15 °C. It grew aerobically and was not capable of anaerobic growth by fermentation of d -glucose or other carbohydrates. Ubiquinone 8 was the predominant isoprenoid quinone. The major polar lipids comprised phosphatidylethanolamine, diphosphatidylglycerol and dimethylaminoethanol. Cellular fatty acids were dominated by C 16:1 ω7c (35.2%), C 16:0 (19.5%), C 18:1 ω7c (18.8%) and C 17:0 ω7c cyclo (15.5%). The DNA G + C content was 49.2 mol% evaluated according to the genomic sequencing data. Strain GS1 T shared more than 96.5% 16S rRNA gene sequence similarities with type strains of four Collimonas species (97.2–97.5%), three Glaciimonas species (97.3% for each of the three) and Oxalicibacterium solurbis (96.5%). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain GS1 T formed a stable genus-level clade with type strains of species in the genus Glaciimonas in the family Oxalobacteraceae and GS1 T was an outgroup with respect to these Glaciimonas species. Characteristically, strain GS1 T could be easily distinguished from the recognised Glaciimonas species by exhibition of swimming motility with monotrichous, subpolar flagellum in some of the cells, ability to grow in NaCl at 2% but not at 3% and the distinguishable fatty acid profiles. On the basis of the polyphasic taxonomic data from this study, strain GS1 T is considered to represent a novel species of the genus Glaciimonas , for which the name Glaciimonas soli sp. nov. is proposed. The type strain is GS1 T (= JCM 33275 T = BCRC 81091 T ).