Interactions between activated sludge extracellular polymeric substances and model carrier surfaces in WWTPs: A combination of QCM-D, AFM and XDLVO prediction

To understand the biofilm formation of biofilm-based processes in wastewater treatment plants (WWTPs), the interaction mechanisms between extracted extracellular polymeric substances (EPS) and three model carrier surfaces (i.e., negatively charged hydrophilic silica, positively charged hydrophilic alumina, and neutral charged hydrophobic polystyrene) were investigated employing a laboratory quartz crystal microbalance with dissipation monitoring equipment (QCM-D) and an atomic force microscope (AFM). The data suggested that surface charge and hydrophobicity of both EPS and carriers played significant roles in the interaction behaviors. Moreover, increases in ionic strength could lead to the increasing zeta potential and hydrophobicity of EPS. It is worth noting that long-range DLVO forces dominated the EPS deposition on carriers in lower ionic strength while short-range Lewis acid-base (AB) interaction controlled the adhesion behaviors in higher ionic strength. Besides, the presence of calcium ions contributed to the adhesion behaviors because of strong charge neutralization and hydrophobic effect. Bound EPS (BEPS) showed higher affinity to model carriers than dissolved EPS (DEPS), which conformed to XDLVO prediction rather than classical DLVO model. Overall, these results provide insights into the influence mechanisms of carrier characteristics, ionic strength, calcium ion and EPS components on the interaction between EPS and representative carriers, contributing to predict and regulate biofilm formation in biofilm-based processes. [Display omitted] •Surface charge & hydrophobicity of EPS and bio-carriers controlled adhesion behaviors.•XDLVO theory predicted QCM-D and AFM results more accurately than DLVO model.•AB interaction rather than EL force dominated the adhesion behaviors under higher IS.•Ca2+ showed stronger charge neutralization and hydrophobic effect on EPS than Na+.•BEPS with higher proteins content showed higher affinity to bio-carriers than DEPS.