Spread of LA-MRSA CC398 in Danish mink (Neovison vison) and mink farm workers

•LA-MRSA CC398 in mink are of the same lineages as the dominant lineages in Danish pigs.•It seems that LA-MRSA CC398 spill over from pigs into farmed mink via contaminated feed.•Mink farmers carry the same lineages of LA-MRSA CC398 as farmed mink and pigs.•Usage of antimicrobials in farmed mink, might challenge eradication of LA-MRSA CC398 in mink. More than 55 million mink skins were produced globally in 2017. As a consequence, a large number of people are employed in mink production worldwide. In Denmark, farmed mink were found to constitute a reservoir of methicillin-resistant Staphylococcus aureus (MRSA) clonal complex (CC) 398 and 6000 mink farm workers in Denmark are potentially exposed to LA-MRSA CC398. The study aim was to elucidate the source of LA-MRSA CC398 in mink farms and to investigate possible transmission to humans. In total, 161 LA-MRSA CC398 isolates from mink (n = 65), mink feed (n = 16) and humans (n = 80) with reported contact to mink, were whole-genome sequenced and compared to 183 LA-MRSA CC398 isolates from Danish pigs and an international collection of 89 S. aureus CC398 isolates. Most of the mink-associated isolates clustered within the predominant LA-MRSA CC398 lineages circulating in the Danish pig production, supporting that pigs are a source of LA-MRSA CC398 in mink feed, mink, and mink farmers.