Effect of heat inactivation of blood samples on the efficacy of three detection methods of SARS-CoV-2 antibodies
To evaluate the effects of heat inactivation of blood samples at 56℃ for 30 min on the results of SARS-CoV-2 antibody detection using different methods. This retrospective study was conducted in 11 patients with established diagnosis of COVID-19 and 10 patients with diseases other than COVID- 19 in...
Gespeichert in:
| Veröffentlicht in: | Nan fang yi ke da xue xue bao = Journal of Southern Medical University 2020-03, Vol.40 (3), p.316-320 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | chi |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 320 |
|---|---|
| container_issue | 3 |
| container_start_page | 316 |
| container_title | Nan fang yi ke da xue xue bao = Journal of Southern Medical University |
| container_volume | 40 |
| creator | Xue, Xiongyan Zhu, Changlin Huang, Shaozhen Pan, Lianhua Xu, Jianhua Li, Weixuan |
| description | To evaluate the effects of heat inactivation of blood samples at 56℃ for 30 min on the results of SARS-CoV-2 antibody detection using different methods.
This retrospective study was conducted in 11 patients with established diagnosis of COVID-19 and 10 patients with diseases other than COVID- 19 in our hospital. We collected samples of serum, plasma and whole blood from each patient between February, 12 and 18, 2020, and with a double- blind design, the samples were examined for SARS-CoV-2 antibodies before and after heat inactivation at 56 ℃ for 30 min. In all the samples, the total SARS-CoV-2 antibodies were detected using immunochromatography, and the IgM antibodies were detected using fluorescence immunochromatography; the IgM and IgG antibodies in the serum and plasma samples detected with chemiluminescence immunoassay. We compared the detection results and analyzed the correlation of semi-quantitative detection results of IgM and IgG antibodies before and after heat inactivation of the samples.
With immuno-chromatography, the coincidence rate of the total SARS-CoV-2 antibody detection before and after heat inactivation of the serum and plasma samples was 90.0% in COVID-19 cases and 100.0% in the negative cases, resulting in a total coincidence rate 95.2%; for the whole blood samples, the total coincidence rates of the total SARS-CoV-2 antibodies were 100.0%. For detection of IgM antibodies in the serum, plasma and whole blood samples using fluorescence immunochromatography, the coincidence rates in SARS-CoV-2-positive and negative cases and the total coincidence rate before and after inactivation were 100.0%, 0 and 47.6%, respectively. For detection of serum IgM and IgG antibodies and plasma IgG antibodies with chemiluminescence immunoassay, the coincidence rates in SARS-CoV-2-positive and negative cases and the total coincidence rate before and after inactivation were all 100.0%, and the total coincidence rate of plasma IgM antibodies was 95.2%. Pearson correlation analysis of the semi-quantitative results of IgM and IgG detection in the serum and plasma samples showed a correlation coefficient of 0.9999 (95%
: 0.9998-1.000,
< 0.001) between the results before and after sample inactivation.
Heat inactivation of blood samples at 56 ℃ for 30 min does not obviously affect the results of immunochromatography and chemiluminescent immunoassay for detection of SARS-COV-2 antibodies but can reduce the risk of infection for the operators. Heat-inactivated sa |
| doi_str_mv | 10.12122/j.issn.1673-4254.2020.03.03 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_2399830634</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2399830634</sourcerecordid><originalsourceid>FETCH-LOGICAL-c1151-a2017b047f0f9aefa14b044df1ddf571f7087c3b61c94f0d3c82930a96420d1a3</originalsourceid><addsrcrecordid>eNo9kE1LxDAQhnNQ3GXdvyA5ePDSmq822-OyrB-wILjqtaTJhEbapjapsP_eFldhYJhn3nl5GYRuKUkpo4zdf6YuhC6lueSJYJlIGWEkJXyqC7T8xwu0DsFVJKNckiwnV2jBGZd5JukS9XtrQUfsLa5BRew6paP7VtH5boZV473BQbV9AwFPLNaAwVqnlT7NglgPANhAnFzmmxZi7U2YV8ft6zHZ-Y-EYdVFV3njIFyjS6uaAOtzX6H3h_3b7ik5vDw-77aHRFOa0UQxQmVFhLTEFgqsomKahLHUGDslt5JspOZVTnUhLDFcb1jBiSpywYihiq_Q3a9vP_ivEUIsWxc0NI3qwI-hZLwoNpzkXEzSm7N0rFowZT-4Vg2n8u9L_AdRYWsb</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2399830634</pqid></control><display><type>article</type><title>Effect of heat inactivation of blood samples on the efficacy of three detection methods of SARS-CoV-2 antibodies</title><source>MEDLINE</source><source>PubMed Central</source><creator>Xue, Xiongyan ; Zhu, Changlin ; Huang, Shaozhen ; Pan, Lianhua ; Xu, Jianhua ; Li, Weixuan</creator><creatorcontrib>Xue, Xiongyan ; Zhu, Changlin ; Huang, Shaozhen ; Pan, Lianhua ; Xu, Jianhua ; Li, Weixuan</creatorcontrib><description>To evaluate the effects of heat inactivation of blood samples at 56℃ for 30 min on the results of SARS-CoV-2 antibody detection using different methods.
This retrospective study was conducted in 11 patients with established diagnosis of COVID-19 and 10 patients with diseases other than COVID- 19 in our hospital. We collected samples of serum, plasma and whole blood from each patient between February, 12 and 18, 2020, and with a double- blind design, the samples were examined for SARS-CoV-2 antibodies before and after heat inactivation at 56 ℃ for 30 min. In all the samples, the total SARS-CoV-2 antibodies were detected using immunochromatography, and the IgM antibodies were detected using fluorescence immunochromatography; the IgM and IgG antibodies in the serum and plasma samples detected with chemiluminescence immunoassay. We compared the detection results and analyzed the correlation of semi-quantitative detection results of IgM and IgG antibodies before and after heat inactivation of the samples.
With immuno-chromatography, the coincidence rate of the total SARS-CoV-2 antibody detection before and after heat inactivation of the serum and plasma samples was 90.0% in COVID-19 cases and 100.0% in the negative cases, resulting in a total coincidence rate 95.2%; for the whole blood samples, the total coincidence rates of the total SARS-CoV-2 antibodies were 100.0%. For detection of IgM antibodies in the serum, plasma and whole blood samples using fluorescence immunochromatography, the coincidence rates in SARS-CoV-2-positive and negative cases and the total coincidence rate before and after inactivation were 100.0%, 0 and 47.6%, respectively. For detection of serum IgM and IgG antibodies and plasma IgG antibodies with chemiluminescence immunoassay, the coincidence rates in SARS-CoV-2-positive and negative cases and the total coincidence rate before and after inactivation were all 100.0%, and the total coincidence rate of plasma IgM antibodies was 95.2%. Pearson correlation analysis of the semi-quantitative results of IgM and IgG detection in the serum and plasma samples showed a correlation coefficient of 0.9999 (95%
: 0.9998-1.000,
< 0.001) between the results before and after sample inactivation.
Heat inactivation of blood samples at 56 ℃ for 30 min does not obviously affect the results of immunochromatography and chemiluminescent immunoassay for detection of SARS-COV-2 antibodies but can reduce the risk of infection for the operators. Heat-inactivated samples can not be used in fluorescence immunochromatography for SARS-CoV-2 antibody detection.</description><identifier>ISSN: 1673-4254</identifier><identifier>DOI: 10.12122/j.issn.1673-4254.2020.03.03</identifier><identifier>PMID: 32376571</identifier><language>chi</language><publisher>China</publisher><subject>Antibodies, Viral ; Betacoronavirus ; Coronavirus Infections - diagnosis ; COVID-19 ; Enzyme-Linked Immunosorbent Assay ; Hot Temperature ; Humans ; Immunoglobulin G ; Immunoglobulin M ; Pandemics ; Pneumonia, Viral - diagnosis ; Retrospective Studies ; SARS-CoV-2</subject><ispartof>Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2020-03, Vol.40 (3), p.316-320</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c1151-a2017b047f0f9aefa14b044df1ddf571f7087c3b61c94f0d3c82930a96420d1a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32376571$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xue, Xiongyan</creatorcontrib><creatorcontrib>Zhu, Changlin</creatorcontrib><creatorcontrib>Huang, Shaozhen</creatorcontrib><creatorcontrib>Pan, Lianhua</creatorcontrib><creatorcontrib>Xu, Jianhua</creatorcontrib><creatorcontrib>Li, Weixuan</creatorcontrib><title>Effect of heat inactivation of blood samples on the efficacy of three detection methods of SARS-CoV-2 antibodies</title><title>Nan fang yi ke da xue xue bao = Journal of Southern Medical University</title><addtitle>Nan Fang Yi Ke Da Xue Xue Bao</addtitle><description>To evaluate the effects of heat inactivation of blood samples at 56℃ for 30 min on the results of SARS-CoV-2 antibody detection using different methods.
This retrospective study was conducted in 11 patients with established diagnosis of COVID-19 and 10 patients with diseases other than COVID- 19 in our hospital. We collected samples of serum, plasma and whole blood from each patient between February, 12 and 18, 2020, and with a double- blind design, the samples were examined for SARS-CoV-2 antibodies before and after heat inactivation at 56 ℃ for 30 min. In all the samples, the total SARS-CoV-2 antibodies were detected using immunochromatography, and the IgM antibodies were detected using fluorescence immunochromatography; the IgM and IgG antibodies in the serum and plasma samples detected with chemiluminescence immunoassay. We compared the detection results and analyzed the correlation of semi-quantitative detection results of IgM and IgG antibodies before and after heat inactivation of the samples.
With immuno-chromatography, the coincidence rate of the total SARS-CoV-2 antibody detection before and after heat inactivation of the serum and plasma samples was 90.0% in COVID-19 cases and 100.0% in the negative cases, resulting in a total coincidence rate 95.2%; for the whole blood samples, the total coincidence rates of the total SARS-CoV-2 antibodies were 100.0%. For detection of IgM antibodies in the serum, plasma and whole blood samples using fluorescence immunochromatography, the coincidence rates in SARS-CoV-2-positive and negative cases and the total coincidence rate before and after inactivation were 100.0%, 0 and 47.6%, respectively. For detection of serum IgM and IgG antibodies and plasma IgG antibodies with chemiluminescence immunoassay, the coincidence rates in SARS-CoV-2-positive and negative cases and the total coincidence rate before and after inactivation were all 100.0%, and the total coincidence rate of plasma IgM antibodies was 95.2%. Pearson correlation analysis of the semi-quantitative results of IgM and IgG detection in the serum and plasma samples showed a correlation coefficient of 0.9999 (95%
: 0.9998-1.000,
< 0.001) between the results before and after sample inactivation.
Heat inactivation of blood samples at 56 ℃ for 30 min does not obviously affect the results of immunochromatography and chemiluminescent immunoassay for detection of SARS-COV-2 antibodies but can reduce the risk of infection for the operators. Heat-inactivated samples can not be used in fluorescence immunochromatography for SARS-CoV-2 antibody detection.</description><subject>Antibodies, Viral</subject><subject>Betacoronavirus</subject><subject>Coronavirus Infections - diagnosis</subject><subject>COVID-19</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Hot Temperature</subject><subject>Humans</subject><subject>Immunoglobulin G</subject><subject>Immunoglobulin M</subject><subject>Pandemics</subject><subject>Pneumonia, Viral - diagnosis</subject><subject>Retrospective Studies</subject><subject>SARS-CoV-2</subject><issn>1673-4254</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE1LxDAQhnNQ3GXdvyA5ePDSmq822-OyrB-wILjqtaTJhEbapjapsP_eFldhYJhn3nl5GYRuKUkpo4zdf6YuhC6lueSJYJlIGWEkJXyqC7T8xwu0DsFVJKNckiwnV2jBGZd5JukS9XtrQUfsLa5BRew6paP7VtH5boZV473BQbV9AwFPLNaAwVqnlT7NglgPANhAnFzmmxZi7U2YV8ft6zHZ-Y-EYdVFV3njIFyjS6uaAOtzX6H3h_3b7ik5vDw-77aHRFOa0UQxQmVFhLTEFgqsomKahLHUGDslt5JspOZVTnUhLDFcb1jBiSpywYihiq_Q3a9vP_ivEUIsWxc0NI3qwI-hZLwoNpzkXEzSm7N0rFowZT-4Vg2n8u9L_AdRYWsb</recordid><startdate>20200330</startdate><enddate>20200330</enddate><creator>Xue, Xiongyan</creator><creator>Zhu, Changlin</creator><creator>Huang, Shaozhen</creator><creator>Pan, Lianhua</creator><creator>Xu, Jianhua</creator><creator>Li, Weixuan</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20200330</creationdate><title>Effect of heat inactivation of blood samples on the efficacy of three detection methods of SARS-CoV-2 antibodies</title><author>Xue, Xiongyan ; Zhu, Changlin ; Huang, Shaozhen ; Pan, Lianhua ; Xu, Jianhua ; Li, Weixuan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1151-a2017b047f0f9aefa14b044df1ddf571f7087c3b61c94f0d3c82930a96420d1a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2020</creationdate><topic>Antibodies, Viral</topic><topic>Betacoronavirus</topic><topic>Coronavirus Infections - diagnosis</topic><topic>COVID-19</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Hot Temperature</topic><topic>Humans</topic><topic>Immunoglobulin G</topic><topic>Immunoglobulin M</topic><topic>Pandemics</topic><topic>Pneumonia, Viral - diagnosis</topic><topic>Retrospective Studies</topic><topic>SARS-CoV-2</topic><toplevel>online_resources</toplevel><creatorcontrib>Xue, Xiongyan</creatorcontrib><creatorcontrib>Zhu, Changlin</creatorcontrib><creatorcontrib>Huang, Shaozhen</creatorcontrib><creatorcontrib>Pan, Lianhua</creatorcontrib><creatorcontrib>Xu, Jianhua</creatorcontrib><creatorcontrib>Li, Weixuan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Nan fang yi ke da xue xue bao = Journal of Southern Medical University</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xue, Xiongyan</au><au>Zhu, Changlin</au><au>Huang, Shaozhen</au><au>Pan, Lianhua</au><au>Xu, Jianhua</au><au>Li, Weixuan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of heat inactivation of blood samples on the efficacy of three detection methods of SARS-CoV-2 antibodies</atitle><jtitle>Nan fang yi ke da xue xue bao = Journal of Southern Medical University</jtitle><addtitle>Nan Fang Yi Ke Da Xue Xue Bao</addtitle><date>2020-03-30</date><risdate>2020</risdate><volume>40</volume><issue>3</issue><spage>316</spage><epage>320</epage><pages>316-320</pages><issn>1673-4254</issn><abstract>To evaluate the effects of heat inactivation of blood samples at 56℃ for 30 min on the results of SARS-CoV-2 antibody detection using different methods.
This retrospective study was conducted in 11 patients with established diagnosis of COVID-19 and 10 patients with diseases other than COVID- 19 in our hospital. We collected samples of serum, plasma and whole blood from each patient between February, 12 and 18, 2020, and with a double- blind design, the samples were examined for SARS-CoV-2 antibodies before and after heat inactivation at 56 ℃ for 30 min. In all the samples, the total SARS-CoV-2 antibodies were detected using immunochromatography, and the IgM antibodies were detected using fluorescence immunochromatography; the IgM and IgG antibodies in the serum and plasma samples detected with chemiluminescence immunoassay. We compared the detection results and analyzed the correlation of semi-quantitative detection results of IgM and IgG antibodies before and after heat inactivation of the samples.
With immuno-chromatography, the coincidence rate of the total SARS-CoV-2 antibody detection before and after heat inactivation of the serum and plasma samples was 90.0% in COVID-19 cases and 100.0% in the negative cases, resulting in a total coincidence rate 95.2%; for the whole blood samples, the total coincidence rates of the total SARS-CoV-2 antibodies were 100.0%. For detection of IgM antibodies in the serum, plasma and whole blood samples using fluorescence immunochromatography, the coincidence rates in SARS-CoV-2-positive and negative cases and the total coincidence rate before and after inactivation were 100.0%, 0 and 47.6%, respectively. For detection of serum IgM and IgG antibodies and plasma IgG antibodies with chemiluminescence immunoassay, the coincidence rates in SARS-CoV-2-positive and negative cases and the total coincidence rate before and after inactivation were all 100.0%, and the total coincidence rate of plasma IgM antibodies was 95.2%. Pearson correlation analysis of the semi-quantitative results of IgM and IgG detection in the serum and plasma samples showed a correlation coefficient of 0.9999 (95%
: 0.9998-1.000,
< 0.001) between the results before and after sample inactivation.
Heat inactivation of blood samples at 56 ℃ for 30 min does not obviously affect the results of immunochromatography and chemiluminescent immunoassay for detection of SARS-COV-2 antibodies but can reduce the risk of infection for the operators. Heat-inactivated samples can not be used in fluorescence immunochromatography for SARS-CoV-2 antibody detection.</abstract><cop>China</cop><pmid>32376571</pmid><doi>10.12122/j.issn.1673-4254.2020.03.03</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1673-4254 |
| ispartof | Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2020-03, Vol.40 (3), p.316-320 |
| issn | 1673-4254 |
| language | chi |
| recordid | cdi_proquest_miscellaneous_2399830634 |
| source | MEDLINE; PubMed Central |
| subjects | Antibodies, Viral Betacoronavirus Coronavirus Infections - diagnosis COVID-19 Enzyme-Linked Immunosorbent Assay Hot Temperature Humans Immunoglobulin G Immunoglobulin M Pandemics Pneumonia, Viral - diagnosis Retrospective Studies SARS-CoV-2 |
| title | Effect of heat inactivation of blood samples on the efficacy of three detection methods of SARS-CoV-2 antibodies |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-10T16%3A37%3A01IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effect%20of%20heat%20inactivation%20of%20blood%20samples%20on%20the%20efficacy%20of%20three%20detection%20methods%20of%20SARS-CoV-2%20antibodies&rft.jtitle=Nan%20fang%20yi%20ke%20da%20xue%20xue%20bao%20=%20Journal%20of%20Southern%20Medical%20University&rft.au=Xue,%20Xiongyan&rft.date=2020-03-30&rft.volume=40&rft.issue=3&rft.spage=316&rft.epage=320&rft.pages=316-320&rft.issn=1673-4254&rft_id=info:doi/10.12122/j.issn.1673-4254.2020.03.03&rft_dat=%3Cproquest_pubme%3E2399830634%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2399830634&rft_id=info:pmid/32376571&rfr_iscdi=true |