Role of biochar and Eisenia fetida on metal bioavailability and biochar effects on earthworm fitness

Biochar has gained extensive attention due to its remediation role in soil pollution. However, its hazardous effects on the soil fauna in contaminated soil and its remediation efficiency affected by soil organisms are still obscure. The individual and combined effects of biochar and earthworms (Eisenia fetida) on soil properties, metal bioavailability, and earthworm fitness were investigated in historically heavy metal (HM)-contaminated soil. The results showed that biochar increased the soil pH by 0.31, decreased DTPA-extractable Cd, Cu, Zn and Pb contents by 11.9%, 14.3%, 5.27% and 23.8%, respectively, and immobilized the HMs from a bioavailable fraction to a residual fraction. The co-incubation of biochar and E. fetida decreased soil pH by 0.11 and increased DTPA-extractable Cu, Zn, and Pb contents by 3.75%, 20.9% and 4.43%, respectively. The results of the correlation analysis showed that soil pH was significantly negatively correlated with HM bioavailability, and it was a potential factor contributed to this opposite effect. Furthermore, biochar decreased the biomass growth of E. fetida and inhibited the activities of SOD, CAT and GSH in E. fetida by 31.1%, 51.3% and 29.6% after 28 days of incubation. Overall, biochar and E. fetida showed the opposite effects on the soil remediation, and biochar also led to a negative effect on earthworms. These findings provided insights on verifying the actual remediation effects of biochar and its ecological risk in situ soil remediation. [Display omitted] •Soil pH and CEC were increased by biochar but decreased by E. fetida.•Biochar decreased the bioavailability of soil metals while increased by E. fetida.•Soil pH was a potential factor to explain this opposite effect for soil remediation.•Biochar showed a negative impact on E. fetida by suppressing its fitness. Biochar and earthworms influenced the soil remediation oppositely while biochar induced negative effects on earthworms.