An Adjustable pH‐Responsive Drug Delivery System Based on Self‐Assembly Polypeptide‐Modified Mesoporous Silica

In this study, an adjustable pH‐responsive drug delivery system using mesoporous silica nanoparticles (MSNs) as the host materials and the modified polypeptides as the nanovalves is reported. Since the polypeptide can self‐assemble via electrostatic interaction at pH 7.4 and be disassembled by pH changes, the modified poly(l‐lysine) and poly(l‐glutamate) are utilized for pore blocking and opening in the study. Poly(l‐lysine)‐MSN (PLL‐MSN) and poly(l‐glutamate)‐MSN (PLG‐MSN) are synthesized via the ring opening polymerization of N‐carboxyanhydrides onto the surface of mesoporous silica nanoparticles. The successful modification of the polypeptide on MSN is proved by Zeta potential change, X‐ray photoelectron spectroscopy (XPS), solid state NMR, and MALDI‐TOF MS. In vitro simulated dye release studies show that PLL‐MSN and PLG‐MSN can successfully load the dye molecules. The release study shows that the controlled release can be constructed at different pH by adjusting the ratio of PLL‐MSN to PLG‐MSN. Cellular uptake study indicates that the drug is detected in both cytoplasm and nucleus, especially in the nucleus. In vitro cytotoxicity assay indicates that DOX loaded mixture nanoparticles (ratio of PLL‐MSN to PLG‐MSN is 1:1) can be triggered for drug release in HeLa cells, resulting in 88% of cell killing. In this study, a simple, novel but effective strategy is proposed for building pH‐sensitive drug delivery systems based on charged poly(l‐lysine)‐mesoporous silica nanoparticle (MSN) and poly(l‐glutamate)‐MSN. Since the polypeptide with good biocompatibility can self‐assemble via electrostatic interaction at pH 7.4 and be disassembled by pH changes, the modified poly(l‐lysine) and poly(l‐glutamate) are utilized for pore blocking and opening in the study.