Photobiomodulation effect on angiogenic proteins produced and released by dental pulp cells

Objective To verify the photobiomodulation effect on angiogenic proteins produced and released by dental human pulpal fibroblasts (HPFs). Material and methods HPFs were irradiated with 660-nm low-level laser at fluences of 2.5 J/cm 2 and 3.7 J/cm 2 . The control group was not irradiated. MTT, crysta...

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Veröffentlicht in:	Clinical oral investigations 2020-12, Vol.24 (12), p.4343-4354
Hauptverfasser:	Vitor, Luciana Lourenço Ribeiro, Bergamo, Mariel Tavares Oliveira Prado, Lourenço-Neto, Natalino, Sakai, Vivien Thiemy, Oliveira, Rodrigo Cardoso, Cruvinel, Thiago, Rios, Daniela, Garlet, Gustavo Pompermaier, Santos, Carlos Ferreira, Machado, Maria Aparecida Andrade Moreira, Oliveira, Thais Marchini
Format:	Artikel
Sprache:	eng
Schlagworte:	Angiogenesis Angiogenic Proteins Cell viability Cells, Cultured Dental Pulp Dentistry Enzyme-linked immunosorbent assay Female Fibroblast growth factor 2 Fibroblasts Humans Light therapy Medicine Original Article Placenta Placenta Growth Factor Platelet-derived growth factor Protein biosynthesis Protein synthesis Proteins Secretion Vascular endothelial growth factor Vascular Endothelial Growth Factor A
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Zusammenfassung:	Objective To verify the photobiomodulation effect on angiogenic proteins produced and released by dental human pulpal fibroblasts (HPFs). Material and methods HPFs were irradiated with 660-nm low-level laser at fluences of 2.5 J/cm 2 and 3.7 J/cm 2 . The control group was not irradiated. MTT, crystal violet, and ELISA assays respectively verified viability, proliferation, and angiogenic protein (supernatant/lysate) at 6 h, 12 h, and 24 h after photobiomodulation. Capillary-like structure formation assay verified functional role. Two-way ANOVA/Tukey’s test and ANOVA/Bonferroni’s multiple comparisons test respectively verified cell viability/proliferation and intragroup and intergroup comparisons of protein synthesis ( p
ISSN:	1432-6981 1436-3771
DOI:	10.1007/s00784-020-03298-1