In vitro antifungal activity and possible mechanisms of action of chelerythrine

Chelerythrine (CHE) possesses broad pharmacological activities. In this study, the extract of Chelidonium majus L. were characterized by high performance liquid chromatography (HPLC), infrared radiation (IR) spectroscopy and nuclear magnetic resonance (NMR). It was proved that the extract was CHE. T...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Pesticide biochemistry and physiology 2020-03, Vol.164, p.140-148
Hauptverfasser: Wei, Qing-hui, Cui, Dai-zong, Liu, Xue-feng, Chai, Yang-yang, Zhao, Na, Wang, Jue-yu, Zhao, Min
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Chelerythrine (CHE) possesses broad pharmacological activities. In this study, the extract of Chelidonium majus L. were characterized by high performance liquid chromatography (HPLC), infrared radiation (IR) spectroscopy and nuclear magnetic resonance (NMR). It was proved that the extract was CHE. The antifungal activity of CHE against five fungal pathogens of rice was researched in vitro, revealing that CHE inhibited Ustilaginoidea virens (U. virens) and Cochliobolus miyabeanus (C. miyabeanus) with 50% effective concentrations (EC50) of 6.53 × 10−3 mg/mL and 5.62 × 10−3 mg/mL, respectively. When the concentration of CHE was 7.5 × 10−3 mg/mL, the inhibition rate of U. virens reached 56.1%. Moreover, CHE (4 × 10−3 mg/mL) exhibited the greatest efficacy in inhibiting spore of U. virens growth with an inhibition rate as high as 86.7%. CHE displayed the best inhibitory activity against U. virens at the concentration of 7.5 × 10−3 mg/mL, compared with the other two isoquinoline alkaloids and commercial fungicide validamycin. After treating U. virens mycelia with CHE, twisted and atrophied mycelia were observed by optical microscopy. SEM results demonstrated narrow and locally fractured mycelium. TEM observations showed that the cell wall had become thin and broken, and most organelles were difficult to recognize. Furthermore, membrane of mycelia was destroyed and reactive oxygen species (ROS) of spores was accumulated, which induced apoptosis of pathogenic fungi. From these results, our understanding of the mechanisms of antifungal activity of CHE against U. virens was enriched and this research is relevant for developing novel pesticides. [Display omitted] •Extracts of CHE were characterized by HPLC, IR spectroscopy and NMR.•CHE has the advantages of low dosage and environmental security for controlling phytopathogenic fungi.•After treating with CHE, morphology of mycelia and spores of U.virens were changed.•CHE might destroy membrane and the ROS steady-state of U. virens.
ISSN:0048-3575
1095-9939
DOI:10.1016/j.pestbp.2020.01.007