Structure of a heptose-containing polysaccharide derived from Komagataeibacter europaeus NBRC 3261

An O-antigen polysaccharide fraction (Ke-PS) was isolated from Komagataeibacter europaeus NBRC 3261. In addition to chemical analyses, its structure was characterized by 1D and 2D 1H as well as 13C NMR spectroscopy. The polysaccharide is composed of linear disaccharide repeating unit that consists of d-galactose and d-glycero-d-manno-heptose: →7)-α-d,d-Hepp-(1 → 2)-α-d-Galp-(1 → . [Display omitted] •High molecular weight polysaccharide in Komagataeibacter europaeus NBRC 3261 was first found.•The structure of the polysaccharide was characterized by chemical analysis and NMR.•The repeating units are novel disaccharides consisting of d-Gal and d,d-Hep.