A fluorescent light-up probe based on AIE and ESIPT processes for β-galactosidase activity detection and visualization in living cells

A novel fluorescent probe SA-βGal is reported here with light-up response to β-galactosidase. SA-βGal possesses the β-galactopyranoside group to react with β-galactosidase and releases the fluorescent salicylaldehyde azine with both aggregation induced emission (AIE) and excited-state intramolecular...

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Veröffentlicht in:Journal of materials chemistry. B, Materials for biology and medicine Materials for biology and medicine, 2015-01, Vol.3 (47), p.9168-9172
Hauptverfasser: Peng, Lu, Gao, Meng, Cai, Xiaolei, Zhang, Ruoyu, Li, Kai, Feng, Guangxue, Tong, Aijun, Liu, Bin
Format: Artikel
Sprache:eng
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Zusammenfassung:A novel fluorescent probe SA-βGal is reported here with light-up response to β-galactosidase. SA-βGal possesses the β-galactopyranoside group to react with β-galactosidase and releases the fluorescent salicylaldehyde azine with both aggregation induced emission (AIE) and excited-state intramolecular proton transfer (ESIPT) characteristics. The linear fluorescent response enables the in vitro quantification of β-galactosidase activity in a range of 0-0.1 U mL with a detection limit of 0.014 U mL . The probe exhibits significant advantages, such as no self-quenching at high concentrations, a large Stokes shift (190 nm) and high specificity to β-galactosidase with an excellent light-up ratio of 820 fold. Moreover, thanks to its good retention in living cells, the application of SA-βGal for the imaging of cellular β-galactosidase was also achieved with high contrast.
ISSN:2050-750X
2050-7518
DOI:10.1039/c5tb01938a