Comparative transcriptomic analysis of rhizomes, stems, and leaves of Polygonatum odoratum (Mill.) Druce reveals candidate genes associated with polysaccharide synthesis

[Display omitted] •Potential genes involved in polysaccharide synthesis were identified in P. odoratum.•76,714 unigenes were annotated in public databases.•KEGG analysis showed 18 key enzymes responsible for polysaccharide biosynthesis.•The most of the upregulated expressed unigenes were enriched in...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Gene 2020-06, Vol.744, p.144626-144626, Article 144626
Hauptverfasser: Zhang, Shengxiang, Shi, Yuanyuan, Huang, Luqi, Wang, Chenkai, Zhao, Derui, Ma, Kelong, Wu, Jiawen, Peng, Daiyin
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:[Display omitted] •Potential genes involved in polysaccharide synthesis were identified in P. odoratum.•76,714 unigenes were annotated in public databases.•KEGG analysis showed 18 key enzymes responsible for polysaccharide biosynthesis.•The most of the upregulated expressed unigenes were enriched in rhizome tissue.•73 TFs involved in polysaccharide synthesis were predicted. Polygonatum odoratum (Mill.) Druce is a well-known traditional Chinese herb. Polysaccharides are major bioactive components of Polygonatum odoratum, which can improve immunity, and are used to treat rheumatic heart disease, cardiovascular disease, and diabetes. This study identified potential genes and transcription factors (TFs) that regulate polysaccharide synthesis in Polygonatum odoratum (Mill.) Druce using RNA sequencing data from leaf, stem, and rhizome tissues. 76,714 unigenes were annotated in public databases. Analysis of KEGG annotations identified 18 key enzymes responsible for polysaccharide biosynthesis and the most of the upregulated expressed unigenes were enriched in rhizome tissue compared with leaf or stem tissue. 73 TFs involved in polysaccharide synthesis were predicted. In addition, key enzyme genes were verified by quantitative real-time PCR. This study substantially enlarged the public transcriptome datasets of this species, and provided insight into detection of novel genes involved in synthesis of polysaccharides and other secondary metabolites.
ISSN:0378-1119
1879-0038
DOI:10.1016/j.gene.2020.144626