Impact of a controlled culture temperature gradient on mouse embryo development and morphokinetics

Temperature control within the IVF laboratory is an important aspect of a quality control system, helping to reduce environmental stress and ensure good-quality embryo development. Temperature fluctuations are probably more common than expected and the optimal temperature for embryo culture is not k...

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Veröffentlicht in:Reproductive biomedicine online 2020-04, Vol.40 (4), p.494-499
Hauptverfasser: Walters, Emily A., Brown, Jessica L., Krisher, Rebecca, Voelkel, Steve, Swain, Jason E.
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Sprache:eng
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Zusammenfassung:Temperature control within the IVF laboratory is an important aspect of a quality control system, helping to reduce environmental stress and ensure good-quality embryo development. Temperature fluctuations are probably more common than expected and the optimal temperature for embryo culture is not known. Modern incubators offer the opportunity to examine the impact of culture temperature on preimplantation embryo development while controlling for other variables within the system. The purpose of this study was to examine the effect of a range of temperatures during extended embryo culture on resulting mouse embryo development and morphokinetic timings. Using a single time-lapse incubator with six individual chambers, frozen–thawed one-cell mouse embryos were cultured individually at temperatures adjusted by 0.5°C between chambers to cover the range of 35.0–37.5°C. Resulting blastocyst formation and embryo morphokinetic timings were recorded and compared. Changes in culture temperature had a significant impact on mouse blastocyst development and morphokinetic timings (P 
ISSN:1472-6483
1472-6491
DOI:10.1016/j.rbmo.2019.12.015