Characterization of a recombinant l-ribose isomerase from Mycetocola miduiensis and its application for the production of l-ribulose

•l-Ribulose producing l-ribose isomerase was characterized from Mycetocola miduiensis.•Mm-LRIse showed maximum activity at 40 °C and pH 7.5 in sodium phosphate (50 mM) buffer.•Mm-LRIse showed the highest specific activity (47.40 U mg−1) with l-ribose as substrate as compared to other l-RIs.•It produ...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Enzyme and microbial technology 2020-04, Vol.135, p.109510-109510, Article 109510
Hauptverfasser: Mahmood, Shahid, Iqbal, Muhammad Waheed, Riaz, Tahreem, Hassanin, Hinawi A.M., Zhu, Yingying, Ni, Dawei, Mu, Wanmeng
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:•l-Ribulose producing l-ribose isomerase was characterized from Mycetocola miduiensis.•Mm-LRIse showed maximum activity at 40 °C and pH 7.5 in sodium phosphate (50 mM) buffer.•Mm-LRIse showed the highest specific activity (47.40 U mg−1) with l-ribose as substrate as compared to other l-RIs.•It produced 32 % l-ribulose as the sole product from l-ribose. An enzyme, l-ribose isomerase (l-RI), mostly catalyzes the isomerization of l-ribose and l-ribulose. These so-called rare sugars are essential for the treatment of cancer and other viral diseases. In the present study, l-ribose isomerase produced from a bacterium, Mycetocola miduiensis (Mm-LRIse), by using l-ribose as a carbon source. The recombinant l-ribose isomerase gene was cloned and overexpressed from M. miduiensis and purified with an exclusive band of 32 kDa by nickel-affinity chromatography. This gene possessed 267 amino acids protein having an estimated molecular weight of 29,568.17 Da. The native molecular weight of Mm-LRIse estimated by HPLC was 134.84 kDa. The recombinant l-ribose isomerase was highly active in sodium phosphate (50 mM) buffer at 40 °C and pH 7.5, showing the specific activity up to 47.40 U mg−1. Mm-LRIse showed no significant enhancement in activity with metallic ions except Mn2+ and Co2+. The values of Km, Kcat, Kcat/Km and Vmax of Mm-LRIse against l-ribose substrate were 42.48 mM, 9259.26 min−1, 217.43 min−1 mM−1, and 277.78 U mg−1 respectively. At equilibrium, the l-ribulose transformation rate was nearly 32 % (6.34 g L−1) using 20 g L−1 of l-ribose. The results revealed that the Mm-LRIse enzyme has a potential for L-ribulose production from l-ribose.
ISSN:0141-0229
1879-0909
DOI:10.1016/j.enzmictec.2020.109510