The eukaryotic tRNA-guanine transglycosylase enzyme inserts queuine into tRNA via a sequential bi-bi mechanism

The eukaryotic tRNA-guanine transglycosylase enzyme inserts queuine into tRNA via a sequential bi-bi mechanism

Eukaryotic tRNA-guanine transglycosylase (TGT) - an enzyme recently recognised to be of potential therapeutic importance - catalyses base-exchange of guanine for queuine at the wobble position of tRNAs associated with 4 amino acids via a distinct mechanism to that reported for its eubacterial homolo...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2020-04, Vol.56 (27), p.3915-3918
Hauptverfasser: Alqasem, Mashael A., Fergus, Claire, Southern, J. Mike, Connon, Stephen J., Kelly, Vincent P.
Format: Artikel
Sprache:eng
Schlagworte:
Amino acids
Chemistry
Chemistry, Multidisciplinary
Enzymes
Homology
Inserts
NMR
Nuclear magnetic resonance
Physical Sciences
Queues
Science & Technology
Substrates
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Beschreibung
Zusammenfassung:Eukaryotic tRNA-guanine transglycosylase (TGT) - an enzyme recently recognised to be of potential therapeutic importance - catalyses base-exchange of guanine for queuine at the wobble position of tRNAs associated with 4 amino acids via a distinct mechanism to that reported for its eubacterial homologue. The presence of queuine is unequivocally required as a trigger for reaction between the enzyme and tRNA and exhibits cooperativity not seen using guanine as a substrate.
ISSN:1359-7345
1364-548X
DOI:10.1039/c9cc09887a