Intestinal carriage of colistin resistant Enterobacteriaceae in hospitalized patients from an Indian center

There is limited data on the gut colonization rate of colistin resistant (Col-R) bacteria in patients and healthy volunteers in India. Aim of this study was to investigate the stool carriage rate of Col-R in hospitalized patients. Stool samples were inoculated in Eosin Methylene Blue agar plates supplemented with colistin. Colistin minimum inhibitory concentrations (MICs) were determined by the broth microdilution method. PCR for the mcr-1 was performed on Col-R Enterobacteriaceae isolates. Mutations in the mgrB gene were analyzed in K. pneumoniae isolates. Mcr-1 positive E. coli was subjected to whole-genome sequencing. Out of 65 stool samples screened, 33 (51%) samples carried Col-R bacteria. Majority (76.7%) of the isolates were sensitive to carbapenem. •Fifty one percent of stool samples from hospitalized patients carried Col-R bacteria.•Majority of the Col-R isolates were sensitive to carbapenem and other tested antibiotics.•mcr-1 was identified in 2 E. coli isolates.•Insertional inactivation of mgrB identified in 2 Klebisella isolates.