Production of l-α-glycerophosphate oxidase by lactic acid bacteria

Various strains of lactic acid bacteria were screened for α-glycerophosphate oxidase activity (GPO). All tested strains of A. viridans and P. acidilactici gave positive results. For six chosen strains, the optimum time of production of GPO was estimated from growth curves. The best GPO producer, A. viridans CCM 2452, was chosen for optimization of nutrient media composition and for experiments with mutagenesis using UV irradiation. Mutant strains were screened for GPO activity. The best one, A. viridans 1509, exhibited good producing stability, and the yield of GPO after modification of cultivation conditions achieved more than 250% of GPO produced by the parent strain under the original cultivation conditions.