Comparison of different mathematical models to assess seasonal variations in the longevity of DNA integrity of cooled‐stored stallion sperm

Dynamic assessment of sperm DNA fragmentation (SDF) has shown to give fuller understanding of stallion semen quality; however, there have been limited attempts to use this parameter to investigate seasonal changes in productive functions. The aims of this study were to: (a) establish a reliable mathematical model to describe the longevity of cooled‐stored sperm DNA integrity; (b) to examine the effect of seasonal variations on SDF. Ejaculates were cooled to 5°C, and SDF was analysed after 0, 6 and 24 hr of storage. The coefficient of determination (R2) was calculated after fine‐tuning linear (LIN), exponential (EXP) and second order polynomial (POL) models. R2 was significantly higher (p