Quantitative determination of cavitation formation and sludge flotation in Anammox granules by using a new diffusion-reaction integrated mathematical model

The granulation of anaerobic ammonium oxidation (Anammox) biomass plays a key role in high rate performance of upflow-type Anammox reactors. However, the formation of cavitation inside granules may result in sludge flotation problem, which negatively affects the operation stability. For quantitative evaluation of the Anammox granules flotation in upflow reactors, an integrated mathematical model was formulated based on the principles that the limitation of substrate diffusion would result in bacterial starvation, lysis and subsequently aiding the formation of cavitation in the inner zone of granules. The proposed model is used to investigate the possible mechanism of cavitation formation and granules flotation. The combined modelling and experimental results showed that the model predictions matched well with the actual floating behavior of granules (R2 = 0.83 for settled sludge and 0.76 for floating sludge). Based on the model results, the granule flotation could be divided into three zones namely (i) no-flotation zone (no flotation occurrence), (ii) transition zone (flotation with a part of granules), and (iii) flotation zone (inevitable flotation occurrence). The floating behavior of granules was mainly influenced by granule diameter (2.5–4.5 mm) and substrate concentration (NO2–N, 50–250 mg/L) in the transition zone. The optimum granule diameter to avoid flotation but with excellent settling performance was identified around 2.5 mm. Additionally, the granule size is more sensitivity to flotation than substrate concentration. Hence, controlling the size of granules is more important to alleviate granule flotation in Anammox reactors’ operation. [Display omitted] •A novel model on Anammox granule flotation was developed based on cell lysis and cavitation.•Granule floating behavior can be assessed by substrate concentration and diameter.•Reactor operating conditions must be kept in no-flotation and transition zones.•Flotation potential increases with the rise in cavitation inside Anammox granules.