A sequence-specific plasmonic loop-mediated isothermal amplification assay with orthogonal color readouts enabled by CRISPR Cas12a

A sequence-specific plasmonic loop-mediated isothermal amplification assay with orthogonal color readouts enabled by CRISPR Cas12a

Herein, we introduce a sequence-specific plasmonic loop-mediated isothermal amplification (LAMP) assay with dual, complementary color readouts enabled by CRISPR Cas12a. Using this assay, any double-stranded LAMP amplicon containing a 5'-TTN PAM sequence can be recognized by Cas12a through a spe...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2020-03, Vol.56 (24), p.3536-3538
Hauptverfasser: Zhou, Rongxing, Li, Yongya, Dong, Tianyu, Tang, Yanan, Li, Feng
Format: Artikel
Sprache:eng
Schlagworte:
Assaying
Color
CRISPR
Signal transduction
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Zusammenfassung:Herein, we introduce a sequence-specific plasmonic loop-mediated isothermal amplification (LAMP) assay with dual, complementary color readouts enabled by CRISPR Cas12a. Using this assay, any double-stranded LAMP amplicon containing a 5'-TTN PAM sequence can be recognized by Cas12a through a specific CRISPR RNA. The signal transduction is achieved using two orthogonal plasmonic systems mediated by Cas12a.
ISSN:1359-7345
1364-548X
DOI:10.1039/d0cc00397b