Digestibility of waste aerobic granular sludge from a full-scale municipal wastewater treatment system

Full-scale aerobic granular sludge technology under the trade name Nereda® has been implemented for municipal, as well as industrial wastewater treatment. Owing to the operational reactor procedures, two types of waste aerobic granular sludge can be clearly distinguished: 1) aerobic granular sludge selection discharge (AGS-SD) and 2) aerobic granular sludge mixture (AGS-RTC). This study systematically compared the anaerobic biodegradability of AGS-SD and AGS-RTC under mesophilic conditions. Results were further compared with the anaerobic conversion of waste activated sludge (WAS) as well as primary sludge (PS) from full-scale municipal wastewater treatment plants. Analysis showed similar chemical characteristics for AGS-SD and PS, which were both characterized by a high carbohydrate content (429 ± 21 and 464 ± 15 mg glucose/g VS sludge, respectively), mainly cellulosic fibres. Concurrently, AGS-RTC exhibited chemical properties close to WAS, both characterized by a relatively high protein content, which were individually 498 ± 14 and 389 ± 15 mg/g VS sludge. AGS-SD was characterized by a high biochemical methane potential (BMP) (296 ± 15 mL CH4/g VS substrate), which was similar to that of PS, and remarkably higher than that of AGS-RTC and WAS. Strikingly, the BMP of AGS-RTC (194 ± 10 mL CH4/g VS substrate) was significantly lower than that of WAS (232 ± 11 mL CH4/g VS substrate). Mechanically destroying the compact structure of AGS-RTC only accelerated the methane production rate but did not significantly affect the BMP value. Results indicated that compared to WAS, the proteins and carbohydrates in AGS-RTC were both more resistant to anaerobic bio-degradation, which might be related to the presence of refractory microbial metabolic products in AGS-RTC. [Display omitted] •Anaerobic digestion of Nereda® waste aerobic granular sludge was carried out.•Two Nereda® waste aerobic granular sludge types had distinct digestion kinetics.•High cellulosic fibres content was observed in selection discharge sludge.•Protein and carbohydrate in aerobic granular sludge mixture were hardly digested.•Mechanically destroying granule’s structure did not affect overall digestibility.