Anaerobic co-digestion of Pennisetum hybrid and pig manure: A comparative study of performance and microbial community at different mixture ratio and organic loading rate

To investigate how the changes in performance and the microbial community of the co-digestion system of Pennisetum hybrid and pig manure, two co-digestion systems in a semi-continuous mode were established at different grass:manure mixture ratios (50:50 and 75:25), and at variable organic loading rates (OLRs). The two reactors were in a steady-state at the OLRs of 2.0–5.0 g VS/(L·d), with the specific and volumetric biogas yields of 383.86 ± 65.13 to 574.28 ± 72.04 mL/g VS and 0.87 ± 0.07 to 2.36 ± 0.13 m3/(m3·d), respectively. The co-digestion system with a mixture ratio of 75:25 failed at an OLR of 5.5 g VS/(L⋅d). This failure could be attributed to the accumulation of volatile fatty acids (VFAs) owing to the imbalance between acid-production and -oxidation bacteria. By contrast, the co-digestion system with mixture ratio of 50:50 failed at an OLR of 7.0 g VS/(L⋅d), which was likely due to mechanical issues or improper reactor configuration. The genus Proteiniphilum contributed to the increase in total ammonia nitrogen. These findings provide useful guidance for optimizing co-digestion system, enhancing reactor performance and improving the wastes treatment. •Co-digestion system with 50:50 mixture ratio obtained better stable performance.•Different issues triggered the failure co-digestion process.•Enriched protein-utilizing Proteiniphilum related to the higher ammonia level.•Shift in methanogen could enhance the stability of GP11.