High-level production and high-yield recovery of lactobionic acid by the control of pH and temperature in fermentation of Pseudomonas taetrolens
Lactobionic acid (LBA) was produced by fermentation of Pseudomonas taetrolens . First, to increase the production of LBA by P. taetrolens , we controlled the pH of culture medium by CaCO 3 addition (30 g/L) and then examined the initial lactose concentration ranging from 50 to 200 g/L and the growth...
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Veröffentlicht in: | Bioprocess and biosystems engineering 2020-05, Vol.43 (5), p.937-944 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Lactobionic acid (LBA) was produced by fermentation of
Pseudomonas taetrolens
. First, to increase the production of LBA by
P. taetrolens
, we controlled the pH of culture medium by CaCO
3
addition (30 g/L) and then examined the initial lactose concentration ranging from 50 to 200 g/L and the growth temperature ranging from 20 to 37 °C. Both the LBA production titer (180 g/L) and the productivity (2.5 g/L h) were highest at 200 g/L lactose concentration and 25 °C of cell growth temperature in shake-flask culture. Although the production of LBA (178 g/L) was almost similar during the batch fermentation of
P. taetrolens
using 5 L bioreactor, the LBA productivity highly increased to 4.9 g/L h. The method using ethanol precipitation and ion-exchange chromatography was developed to recover the pure LBA from the fermentation broth. The optimum volume of ethanol and pH of culture medium for the precipitation of Ca
2+
salt form of LBA were six volume of ethanol and pH 6.5, respectively. The cation-exchange resin T42 finally showed the best recovery yield (97.6%) of LBA from the culture supernatant. The production titer (178 g/L) and the productivity (4.9 g/L h) of lactobionic acid in this study were highest among the previous studies ever reported using
P. taetrolens
as a production strain of LBA. |
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ISSN: | 1615-7591 1615-7605 |
DOI: | 10.1007/s00449-020-02290-z |