Development of polyclonal-antibody-coated immunomagnetic beads for separation and detection of koi herpesvirus in large-volume samples

To separate and concentrate koi herpesvirus (KHV) from large-volume samples, a separation method based on immunomagnetic beads (IMBs) coated with polyclonal antibody directed against KHV was developed. After treatment with IMBs, viral DNA was extracted from samples and used as a template for quantit...

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Veröffentlicht in:	Archives of virology 2020-04, Vol.165 (4), p.973-976
Hauptverfasser:	Zhang, Min, Li, Yingying, Jing, Hongli, Wang, Na, Wu, Shaoqiang, Wang, Qing, Lin, Xiangmei
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies - analysis Biomedical and Life Sciences Biomedicine Brief Report Carps - virology Deoxyribonucleic acid DNA Fish Diseases - virology Herpesviridae - genetics Herpesviridae - isolation & purification Herpesviridae Infections - veterinary Herpesviridae Infections - virology Immunomagnetic Separation - instrumentation Immunomagnetic Separation - methods Infectious Diseases Medical Microbiology Polyclonal antibodies Real-Time Polymerase Chain Reaction Sensitivity and Specificity Virology
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Beschreibung
Zusammenfassung:	To separate and concentrate koi herpesvirus (KHV) from large-volume samples, a separation method based on immunomagnetic beads (IMBs) coated with polyclonal antibody directed against KHV was developed. After treatment with IMBs, viral DNA was extracted from samples and used as a template for quantitative PCR (qPCR). The results showed that the concentration of the template DNA extracted from the virus that had been separated using IMBs was 9.65-fold higher than that from virus not treated with IMBs. The detection limit of the IMBs/qPCR method was found to be at least 10 times lower than that of qPCR alone.
ISSN:	0304-8608 1432-8798
DOI:	10.1007/s00705-020-04557-y