Tabrizicola piscis sp. nov., isolated from the intestinal tract of a Korean indigenous freshwater fish, Acheilognathus koreensis

A novel Gram-negative, obligately aerobic, rod-shaped and non-motile bacterium, designated strain K13M18(T), was isolated from the intestinal tract of a Korean indigenous fish, oily bitterling (Acheilognathus koreensis). Strain K13M18(T) formed creamypink colonies on a marine agar plate. Results of phylogenetic analysis based on the 16S rRNA gene sequence similarity indicated that strain K13M18(T) was most closely related to Tabrizicola sediminis DRYC-M-16(T), sharing 97.62 % similarity with that strain. Strain K13M18(T) belonged to the genus Tabrizicola, which formed a cluster with Tabrizicola aquatica RCRI19(T), Tabrizicola fusiformis SY72(T), Tabrizicola sediminis DRYC-M-16(T) and Tabrizicola alkalilacus DJC(T) in a phylogenetic tree based on the 16S rRNA gene sequences. Strain K13M18(T) grown optimally in 0 % (w/v) NaCl, at pH 7 and 30 degrees C, in a marine broth medium. The predominant cellular fatty acids were C-18:0 omega 7c and C-18:(1)omega 6c. The major respiratory isoprenoid quinone was ubiquinone 0-10. Polar lipids of strain K13M18(T) contained phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol, diphosphatidylglycerol, six unidentified aminophospholipids, one unidentified aminolipid and an unidentified lipid. Based on genome sequencing, the DNA G+C content of strain K13M18(T) was 64.08 mol %, with an average nucleotide identity value, calculated by a comparative genomic analysis of strains K13M18(T) and T. sediminis DRYC-M-16(T), of 74.82 %. Based on the phylogenetic, genotypic, and phenotypic information, strain K13M18(T) is proposed to be a novel species of the genus Tabrizicola. The type strain is K13M18(T) (=KCTC 62659(T)=JCM 33230(T)).