Effects of recombinant Lactobacillus casei on growth performance, immune response and disease resistance in crucian carp, Carassius auratus

Effects of recombinant Lactobacillus casei on growth performance, immune response and disease resistance in crucian carp, Carassius auratus

In the present study, we constructed two recombinant Lactobacillus casei (L. casei) Lc-pPG-1-AcrV (surface-displayed) and Lc-pPG-2-AcrV (secretory) constitutively expressing AcrV protein of Aeromonas veronii (A. veronii). Expression of recombinant AcrV protein was verified by western blot and immuno...

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Veröffentlicht in:Fish & shellfish immunology 2020-04, Vol.99, p.73-85
Hauptverfasser: Kong, Yidi, Li, Min, Tian, Jiaxin, Zhao, Linhui, Kang, Yuanhuan, Zhang, Lei, Wang, Guiqin, Shan, Xiaofeng
Format: Artikel
Sprache:eng
Schlagworte:
Crucian carp
Disease resistance
Immune response
Lactobacillus casei
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Zusammenfassung:In the present study, we constructed two recombinant Lactobacillus casei (L. casei) Lc-pPG-1-AcrV (surface-displayed) and Lc-pPG-2-AcrV (secretory) constitutively expressing AcrV protein of Aeromonas veronii (A. veronii). Expression of recombinant AcrV protein was verified by western blot and immunofluorescence technique. Compared with PBS group, the final weight (FW), weight gain (WG) and specific growth rate (SGR) of fish fed Lc-pPG-1-AcrV, Lc-pPG-2-AcrV and Lc-pPG diets after 56 days observed significantly increase (p 
ISSN:1050-4648
1095-9947
DOI:10.1016/j.fsi.2020.02.008