Manipulating regioselectivity of an epoxide hydrolase for single enzymatic synthesis of ()-1,2-diols from racemic epoxides

Both the activity and regioselectivity of Phaseolus vulgaris epoxide hydrolase were remarkably improved via reshaping two substrate tunnels based on rational design. The elegant one-step enantioconvergent hydrolysis of seven rac -epoxides was achieved by single mutants, allowing green and efficient access to valuable ( R )-1,2 diols with high ee p (90.1-98.3%) and yields. Both the activity and regioselectivity of Phaseolus vulgaris epoxide hydrolase were remarkably improved via reshaping two substrate tunnels based on rational design.