Ultra‐fast Cycling for Multiplexed Cellular Fluorescence Imaging

Ultra‐fast Cycling for Multiplexed Cellular Fluorescence Imaging

Rapid analysis of single and scant cell populations is essential in modern diagnostics, yet existing methods are often limited and slow. Herein, we describe an ultra‐fast, highly efficient cycling method for the analysis of single cells based on unique linkers for tetrazine (Tz)/trans‐cyclooctene (T...

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Veröffentlicht in:Angewandte Chemie International Edition 2020-04, Vol.59 (17), p.6839-6846
Hauptverfasser: Ko, Jina, Oh, Juhyun, Ahmed, Maaz S., Carlson, Jonathan C. T., Weissleder, Ralph
Format: Artikel
Sprache:eng
Schlagworte:
bioorthogonal chemistry
cancer
click chemistry
Cycles
Cyclooctanes - chemistry
Diagnostic systems
Fluorescence
fluorescent probes
HeLa Cells
Humans
Immune system
Kinetics
Optical Imaging - methods
Quenching
Reaction kinetics
Single-Cell Analysis
tetrazines
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Beschreibung
Zusammenfassung:Rapid analysis of single and scant cell populations is essential in modern diagnostics, yet existing methods are often limited and slow. Herein, we describe an ultra‐fast, highly efficient cycling method for the analysis of single cells based on unique linkers for tetrazine (Tz)/trans‐cyclooctene (TCO)‐mediated quenching. Surprisingly, the quenching reaction rates were more than 3 orders of magnitude faster (t1/2
ISSN:1433-7851
1521-3773
1521-3773
DOI:10.1002/anie.201915153