Serratia nevei sp. nov. and Serratia bockelmannii sp. nov., isolated from fresh produce in Germany and reclassification of Serratia marcescens subsp. sakuensis Ajithkumar et al. 2003 as a later heterotypic synonym of Serratia marcescens subsp. marcescens

Fifteen enterobacterial strains were isolated from fresh produce. The 16S rRNA gene sequences indicated that these belong to Serratia, with twelve strains showing 99.57%–99.93% and three strains showing 99.86–100% 16S rRNA gene sequence similarity with Serratia marcescens and Serratia nematodiphila...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Systematic and applied microbiology 2020-03, Vol.43 (2), p.126055-126055, Article 126055
Hauptverfasser: Cho, Gyu-Sung, Stein, Maria, Brinks, Erik, Rathje, Jana, Lee, Woojung, Suh, Soo Hwan, Franz, Charles M.A.P.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 126055
container_issue 2
container_start_page 126055
container_title Systematic and applied microbiology
container_volume 43
creator Cho, Gyu-Sung
Stein, Maria
Brinks, Erik
Rathje, Jana
Lee, Woojung
Suh, Soo Hwan
Franz, Charles M.A.P.
description Fifteen enterobacterial strains were isolated from fresh produce. The 16S rRNA gene sequences indicated that these belong to Serratia, with twelve strains showing 99.57%–99.93% and three strains showing 99.86–100% 16S rRNA gene sequence similarity with Serratia marcescens and Serratia nematodiphila as nearest neighbors, respectively. Further comparative multi locus sequence analyses, as well as phylogenomic comparisons, revealed that 6 of the 15 strains were well-separated from their nearest neighbors and formed two clearly distinct taxa. Strains S2, S9, S10 and S15T were urease-positive, while strains S3T and S13 were urease-negative. Average nucleotide identity and digital DNA–DNA hybridization comparisons of representative strains S3T and S15T with type strains of S. marcescens, S. nematodiphila and S. ureilytica indicated that these shared less than 96% and 70% homology, respectively. Major fatty acids of strains S3T and S15T included C16:0, C16:1 ω7c/C16:1 ω6c, C17:0 Cyclo and C18:1 ω6c /C18:1 ω7c. The mol% G+C of genomic DNA of strain S15T was 59.49% and of strain S3T was 59.04. These results support the description of two novel species, Serratia nevei and Serratia bockelmannii, with strains S15T (=LMG 31536T =DSM 110085T) and S3T (=LMG 31535T =DSM 110152T) as type strains, respectively. Although Serratia marcescens subsp. sakuensis was previously described to form spores, spores could not be determined in this study. As spore formation was the only differential characteristic of this subspecies, S. marcescens subsp. sakuensis is a later heterotypic synonym of Serratia marcescens.
doi_str_mv 10.1016/j.syapm.2020.126055
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2348220803</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0723202020300035</els_id><sourcerecordid>2348220803</sourcerecordid><originalsourceid>FETCH-LOGICAL-c425t-8a01d3fbf1a8d087bcd49b67578cf90442c2adbc9a93f4b358a00c437678a93</originalsourceid><addsrcrecordid>eNqFUUtv1DAQthCIbgu_AAnNkQMJtvM-cKgqaJEqcSh3y3EmWu8m9uJJVsqfr3C6pXuDy1ie-R5jf4x9EDwVXJRfdikt-jCmksvYkSUvildsI0pRJ7yp89dswyuZJev4gl0S7TgXeVOKt-wiE00j86basMcHDEFPVoPDI1qgQwrOH1PQroOXWevNHodRO2fPkM9gyQ96wg764MdYkLZwCL6bDYJ1cIshUpYnqYBm0ES2tyYqege-P8uPOhgkg46A5nbVJ72f49USXO_stN3PEQI4gR5SkJxnoAk0rOYBthirn5aDNUCL824Z_6N-7rxjb3o9EL5_Pq_Yw_dvv27ukvuftz9uru8Tk8tiSmrNRZf1bS903fG6ak2XN21ZFVVt-obnuTRSd61pdJP1eZsVkcBNnlVlVcfWFft0Uo2f83tGmtRoo_0waId-JiWzvJaS1zyL0OwENcETBezVIdi476IEV2vsaqeeYldrruoUe2R9fDaY2xG7F87fnCPg6wmA8ZFHi0GRsegMdjYmM6nO238a_AFgM8T6</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2348220803</pqid></control><display><type>article</type><title>Serratia nevei sp. nov. and Serratia bockelmannii sp. nov., isolated from fresh produce in Germany and reclassification of Serratia marcescens subsp. sakuensis Ajithkumar et al. 2003 as a later heterotypic synonym of Serratia marcescens subsp. marcescens</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Cho, Gyu-Sung ; Stein, Maria ; Brinks, Erik ; Rathje, Jana ; Lee, Woojung ; Suh, Soo Hwan ; Franz, Charles M.A.P.</creator><creatorcontrib>Cho, Gyu-Sung ; Stein, Maria ; Brinks, Erik ; Rathje, Jana ; Lee, Woojung ; Suh, Soo Hwan ; Franz, Charles M.A.P.</creatorcontrib><description>Fifteen enterobacterial strains were isolated from fresh produce. The 16S rRNA gene sequences indicated that these belong to Serratia, with twelve strains showing 99.57%–99.93% and three strains showing 99.86–100% 16S rRNA gene sequence similarity with Serratia marcescens and Serratia nematodiphila as nearest neighbors, respectively. Further comparative multi locus sequence analyses, as well as phylogenomic comparisons, revealed that 6 of the 15 strains were well-separated from their nearest neighbors and formed two clearly distinct taxa. Strains S2, S9, S10 and S15T were urease-positive, while strains S3T and S13 were urease-negative. Average nucleotide identity and digital DNA–DNA hybridization comparisons of representative strains S3T and S15T with type strains of S. marcescens, S. nematodiphila and S. ureilytica indicated that these shared less than 96% and 70% homology, respectively. Major fatty acids of strains S3T and S15T included C16:0, C16:1 ω7c/C16:1 ω6c, C17:0 Cyclo and C18:1 ω6c /C18:1 ω7c. The mol% G+C of genomic DNA of strain S15T was 59.49% and of strain S3T was 59.04. These results support the description of two novel species, Serratia nevei and Serratia bockelmannii, with strains S15T (=LMG 31536T =DSM 110085T) and S3T (=LMG 31535T =DSM 110152T) as type strains, respectively. Although Serratia marcescens subsp. sakuensis was previously described to form spores, spores could not be determined in this study. As spore formation was the only differential characteristic of this subspecies, S. marcescens subsp. sakuensis is a later heterotypic synonym of Serratia marcescens.</description><identifier>ISSN: 0723-2020</identifier><identifier>EISSN: 1618-0984</identifier><identifier>DOI: 10.1016/j.syapm.2020.126055</identifier><identifier>PMID: 31992497</identifier><language>eng</language><publisher>Germany: Elsevier GmbH</publisher><subject>Bacterial Proteins - genetics ; DNA, Bacterial - genetics ; Endospore ; Fatty Acids - analysis ; Food Microbiology ; Genome ; Genome, Bacterial - genetics ; Genotype ; Germany ; Multi locus sequence analysis ; Nucleic Acid Hybridization ; Phenotype ; Phylogeny ; RNA, Ribosomal, 16S - genetics ; Sequence Analysis, DNA ; Serratia ; Serratia - chemistry ; Serratia - classification ; Serratia - genetics ; Serratia - isolation &amp; purification ; Spores, Bacterial - cytology ; Spores, Bacterial - growth &amp; development</subject><ispartof>Systematic and applied microbiology, 2020-03, Vol.43 (2), p.126055-126055, Article 126055</ispartof><rights>2020 Elsevier GmbH</rights><rights>Copyright © 2020 Elsevier GmbH. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c425t-8a01d3fbf1a8d087bcd49b67578cf90442c2adbc9a93f4b358a00c437678a93</citedby><cites>FETCH-LOGICAL-c425t-8a01d3fbf1a8d087bcd49b67578cf90442c2adbc9a93f4b358a00c437678a93</cites><orcidid>0000-0003-2009-7172</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0723202020300035$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31992497$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cho, Gyu-Sung</creatorcontrib><creatorcontrib>Stein, Maria</creatorcontrib><creatorcontrib>Brinks, Erik</creatorcontrib><creatorcontrib>Rathje, Jana</creatorcontrib><creatorcontrib>Lee, Woojung</creatorcontrib><creatorcontrib>Suh, Soo Hwan</creatorcontrib><creatorcontrib>Franz, Charles M.A.P.</creatorcontrib><title>Serratia nevei sp. nov. and Serratia bockelmannii sp. nov., isolated from fresh produce in Germany and reclassification of Serratia marcescens subsp. sakuensis Ajithkumar et al. 2003 as a later heterotypic synonym of Serratia marcescens subsp. marcescens</title><title>Systematic and applied microbiology</title><addtitle>Syst Appl Microbiol</addtitle><description>Fifteen enterobacterial strains were isolated from fresh produce. The 16S rRNA gene sequences indicated that these belong to Serratia, with twelve strains showing 99.57%–99.93% and three strains showing 99.86–100% 16S rRNA gene sequence similarity with Serratia marcescens and Serratia nematodiphila as nearest neighbors, respectively. Further comparative multi locus sequence analyses, as well as phylogenomic comparisons, revealed that 6 of the 15 strains were well-separated from their nearest neighbors and formed two clearly distinct taxa. Strains S2, S9, S10 and S15T were urease-positive, while strains S3T and S13 were urease-negative. Average nucleotide identity and digital DNA–DNA hybridization comparisons of representative strains S3T and S15T with type strains of S. marcescens, S. nematodiphila and S. ureilytica indicated that these shared less than 96% and 70% homology, respectively. Major fatty acids of strains S3T and S15T included C16:0, C16:1 ω7c/C16:1 ω6c, C17:0 Cyclo and C18:1 ω6c /C18:1 ω7c. The mol% G+C of genomic DNA of strain S15T was 59.49% and of strain S3T was 59.04. These results support the description of two novel species, Serratia nevei and Serratia bockelmannii, with strains S15T (=LMG 31536T =DSM 110085T) and S3T (=LMG 31535T =DSM 110152T) as type strains, respectively. Although Serratia marcescens subsp. sakuensis was previously described to form spores, spores could not be determined in this study. As spore formation was the only differential characteristic of this subspecies, S. marcescens subsp. sakuensis is a later heterotypic synonym of Serratia marcescens.</description><subject>Bacterial Proteins - genetics</subject><subject>DNA, Bacterial - genetics</subject><subject>Endospore</subject><subject>Fatty Acids - analysis</subject><subject>Food Microbiology</subject><subject>Genome</subject><subject>Genome, Bacterial - genetics</subject><subject>Genotype</subject><subject>Germany</subject><subject>Multi locus sequence analysis</subject><subject>Nucleic Acid Hybridization</subject><subject>Phenotype</subject><subject>Phylogeny</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Serratia</subject><subject>Serratia - chemistry</subject><subject>Serratia - classification</subject><subject>Serratia - genetics</subject><subject>Serratia - isolation &amp; purification</subject><subject>Spores, Bacterial - cytology</subject><subject>Spores, Bacterial - growth &amp; development</subject><issn>0723-2020</issn><issn>1618-0984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUUtv1DAQthCIbgu_AAnNkQMJtvM-cKgqaJEqcSh3y3EmWu8m9uJJVsqfr3C6pXuDy1ie-R5jf4x9EDwVXJRfdikt-jCmksvYkSUvildsI0pRJ7yp89dswyuZJev4gl0S7TgXeVOKt-wiE00j86basMcHDEFPVoPDI1qgQwrOH1PQroOXWevNHodRO2fPkM9gyQ96wg764MdYkLZwCL6bDYJ1cIshUpYnqYBm0ES2tyYqege-P8uPOhgkg46A5nbVJ72f49USXO_stN3PEQI4gR5SkJxnoAk0rOYBthirn5aDNUCL824Z_6N-7rxjb3o9EL5_Pq_Yw_dvv27ukvuftz9uru8Tk8tiSmrNRZf1bS903fG6ak2XN21ZFVVt-obnuTRSd61pdJP1eZsVkcBNnlVlVcfWFft0Uo2f83tGmtRoo_0waId-JiWzvJaS1zyL0OwENcETBezVIdi476IEV2vsaqeeYldrruoUe2R9fDaY2xG7F87fnCPg6wmA8ZFHi0GRsegMdjYmM6nO238a_AFgM8T6</recordid><startdate>202003</startdate><enddate>202003</enddate><creator>Cho, Gyu-Sung</creator><creator>Stein, Maria</creator><creator>Brinks, Erik</creator><creator>Rathje, Jana</creator><creator>Lee, Woojung</creator><creator>Suh, Soo Hwan</creator><creator>Franz, Charles M.A.P.</creator><general>Elsevier GmbH</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-2009-7172</orcidid></search><sort><creationdate>202003</creationdate><title>Serratia nevei sp. nov. and Serratia bockelmannii sp. nov., isolated from fresh produce in Germany and reclassification of Serratia marcescens subsp. sakuensis Ajithkumar et al. 2003 as a later heterotypic synonym of Serratia marcescens subsp. marcescens</title><author>Cho, Gyu-Sung ; Stein, Maria ; Brinks, Erik ; Rathje, Jana ; Lee, Woojung ; Suh, Soo Hwan ; Franz, Charles M.A.P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c425t-8a01d3fbf1a8d087bcd49b67578cf90442c2adbc9a93f4b358a00c437678a93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Bacterial Proteins - genetics</topic><topic>DNA, Bacterial - genetics</topic><topic>Endospore</topic><topic>Fatty Acids - analysis</topic><topic>Food Microbiology</topic><topic>Genome</topic><topic>Genome, Bacterial - genetics</topic><topic>Genotype</topic><topic>Germany</topic><topic>Multi locus sequence analysis</topic><topic>Nucleic Acid Hybridization</topic><topic>Phenotype</topic><topic>Phylogeny</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Serratia</topic><topic>Serratia - chemistry</topic><topic>Serratia - classification</topic><topic>Serratia - genetics</topic><topic>Serratia - isolation &amp; purification</topic><topic>Spores, Bacterial - cytology</topic><topic>Spores, Bacterial - growth &amp; development</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cho, Gyu-Sung</creatorcontrib><creatorcontrib>Stein, Maria</creatorcontrib><creatorcontrib>Brinks, Erik</creatorcontrib><creatorcontrib>Rathje, Jana</creatorcontrib><creatorcontrib>Lee, Woojung</creatorcontrib><creatorcontrib>Suh, Soo Hwan</creatorcontrib><creatorcontrib>Franz, Charles M.A.P.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Systematic and applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cho, Gyu-Sung</au><au>Stein, Maria</au><au>Brinks, Erik</au><au>Rathje, Jana</au><au>Lee, Woojung</au><au>Suh, Soo Hwan</au><au>Franz, Charles M.A.P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Serratia nevei sp. nov. and Serratia bockelmannii sp. nov., isolated from fresh produce in Germany and reclassification of Serratia marcescens subsp. sakuensis Ajithkumar et al. 2003 as a later heterotypic synonym of Serratia marcescens subsp. marcescens</atitle><jtitle>Systematic and applied microbiology</jtitle><addtitle>Syst Appl Microbiol</addtitle><date>2020-03</date><risdate>2020</risdate><volume>43</volume><issue>2</issue><spage>126055</spage><epage>126055</epage><pages>126055-126055</pages><artnum>126055</artnum><issn>0723-2020</issn><eissn>1618-0984</eissn><abstract>Fifteen enterobacterial strains were isolated from fresh produce. The 16S rRNA gene sequences indicated that these belong to Serratia, with twelve strains showing 99.57%–99.93% and three strains showing 99.86–100% 16S rRNA gene sequence similarity with Serratia marcescens and Serratia nematodiphila as nearest neighbors, respectively. Further comparative multi locus sequence analyses, as well as phylogenomic comparisons, revealed that 6 of the 15 strains were well-separated from their nearest neighbors and formed two clearly distinct taxa. Strains S2, S9, S10 and S15T were urease-positive, while strains S3T and S13 were urease-negative. Average nucleotide identity and digital DNA–DNA hybridization comparisons of representative strains S3T and S15T with type strains of S. marcescens, S. nematodiphila and S. ureilytica indicated that these shared less than 96% and 70% homology, respectively. Major fatty acids of strains S3T and S15T included C16:0, C16:1 ω7c/C16:1 ω6c, C17:0 Cyclo and C18:1 ω6c /C18:1 ω7c. The mol% G+C of genomic DNA of strain S15T was 59.49% and of strain S3T was 59.04. These results support the description of two novel species, Serratia nevei and Serratia bockelmannii, with strains S15T (=LMG 31536T =DSM 110085T) and S3T (=LMG 31535T =DSM 110152T) as type strains, respectively. Although Serratia marcescens subsp. sakuensis was previously described to form spores, spores could not be determined in this study. As spore formation was the only differential characteristic of this subspecies, S. marcescens subsp. sakuensis is a later heterotypic synonym of Serratia marcescens.</abstract><cop>Germany</cop><pub>Elsevier GmbH</pub><pmid>31992497</pmid><doi>10.1016/j.syapm.2020.126055</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0003-2009-7172</orcidid></addata></record>
fulltext fulltext
identifier ISSN: 0723-2020
ispartof Systematic and applied microbiology, 2020-03, Vol.43 (2), p.126055-126055, Article 126055
issn 0723-2020
1618-0984
language eng
recordid cdi_proquest_miscellaneous_2348220803
source MEDLINE; Elsevier ScienceDirect Journals
subjects Bacterial Proteins - genetics
DNA, Bacterial - genetics
Endospore
Fatty Acids - analysis
Food Microbiology
Genome
Genome, Bacterial - genetics
Genotype
Germany
Multi locus sequence analysis
Nucleic Acid Hybridization
Phenotype
Phylogeny
RNA, Ribosomal, 16S - genetics
Sequence Analysis, DNA
Serratia
Serratia - chemistry
Serratia - classification
Serratia - genetics
Serratia - isolation & purification
Spores, Bacterial - cytology
Spores, Bacterial - growth & development
title Serratia nevei sp. nov. and Serratia bockelmannii sp. nov., isolated from fresh produce in Germany and reclassification of Serratia marcescens subsp. sakuensis Ajithkumar et al. 2003 as a later heterotypic synonym of Serratia marcescens subsp. marcescens
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-01T03%3A11%3A01IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Serratia%20nevei%20sp.%20nov.%20and%20Serratia%20bockelmannii%20sp.%20nov.,%20isolated%20from%20fresh%20produce%20in%20Germany%20and%20reclassification%20of%20Serratia%20marcescens%20subsp.%20sakuensis%20Ajithkumar%20et%20al.%202003%20as%20a%20later%20heterotypic%20synonym%20of%20Serratia%20marcescens%20subsp.%20marcescens&rft.jtitle=Systematic%20and%20applied%20microbiology&rft.au=Cho,%20Gyu-Sung&rft.date=2020-03&rft.volume=43&rft.issue=2&rft.spage=126055&rft.epage=126055&rft.pages=126055-126055&rft.artnum=126055&rft.issn=0723-2020&rft.eissn=1618-0984&rft_id=info:doi/10.1016/j.syapm.2020.126055&rft_dat=%3Cproquest_cross%3E2348220803%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2348220803&rft_id=info:pmid/31992497&rft_els_id=S0723202020300035&rfr_iscdi=true