Whole‐Cell Biocatalysis in Seawater: New Halotolerant Yeast Strains for the Regio‐ and Stereoselectivity Reduction of 1‐Phenylpropane‐1,2‐Dione in Saline‐Rich Media

The application of green chemistry concepts in catalysis has considerably increased in recent years, and the interest in using sustainable solvents in the chemical industry is growing. One of the recent proposals to fall in line with this is to employ seawater as a solvent in biocatalytic processes. This involves selecting halotolerant strains capable of carrying out chemical conversions in the presence of the salt concentrations found in this solution. Recent studies by our group have revealed the interest in using strains belonging to Debaryomyces and Schwanniomyces for catalytic processes run in this medium. In the present work, we select other yeasts based on their halotolerance to widen the scope of this strategy. We consider them for the monoreduction of 1‐phenylpropane‐1,2‐dione, a well‐characterized reaction that produces acyloin intermediates of pharmaceutical interest. The results obtained herein indicate that using seawater as a solvent for this reaction is possible. The best ones were obtained for Saccharomyces cerevisiae FY86 and Kluyveromyces marxianus, for which acyloins with different stereochemistry were obtained with good to excellent enantiomeric excess. Whole‐cell biocatalysis in seawater: New halotolerant yeast strains were used to regio‐ and stereoselectively reduce 1‐phenylpropane‐1,2‐dione in sea/freshwater to acyloin intermediates of pharmaceutical interest. This indicates that using seawater as a green solvent for this reaction is possible when large quantities of water are required.