Simultaneous hydrolysis of cheese whey and lactulose production catalyzed by β-galactosidase from Kluyveromyces lactis NRRL Y1564

β-Galactosidase was produced by the yeast Kluyveromyces lactis NRRL Y1564 in cheese whey supplemented with yeast extract under the optimal temperature of 30 °C, delivering an enzymatic activity of 4418.37 U/g cell after 12 h of process. In order to develop more stable biocatalysts, the enzyme produc...

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Veröffentlicht in:Bioprocess and biosystems engineering 2020-04, Vol.43 (4), p.711-722
Hauptverfasser: de Freitas, Maria de Fátima Matos, Hortêncio, Lucas C., de Albuquerque, Tiago Lima, Rocha, Maria Valderez Ponte, Gonçalves, Luciana Rocha Barros
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container_issue 4
container_start_page 711
container_title Bioprocess and biosystems engineering
container_volume 43
creator de Freitas, Maria de Fátima Matos
Hortêncio, Lucas C.
de Albuquerque, Tiago Lima
Rocha, Maria Valderez Ponte
Gonçalves, Luciana Rocha Barros
description β-Galactosidase was produced by the yeast Kluyveromyces lactis NRRL Y1564 in cheese whey supplemented with yeast extract under the optimal temperature of 30 °C, delivering an enzymatic activity of 4418.37 U/g cell after 12 h of process. In order to develop more stable biocatalysts, the enzyme produced by fermentation was immobilized on 2.0% w/v chitosan activated with glutaraldehyde, epichlorohydrin or glycidol, producing a highly active and stable biocatalyst capable of hydrolyzing lactose and producing lactulose simultaneously. The biocatalyst obtained by immobilization in chitosan-glutaraldehyde showed high storage stabilities (100% of its activity when stored at 4 °C 105 days). Regarding the milk lactose hydrolysis by both the soluble and the immobilized enzyme, the conversions obtained were 38.0% and 42.8%, respectively. In this study, by using a biocatalyst deriving from enzyme immobilization to chitosan support, a lactulose production of 17.32 g/L was also possible.
doi_str_mv 10.1007/s00449-019-02270-y
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subjects beta-Galactosidase - chemistry
Biocatalysts
Biotechnology
Cheese
Chemistry
Chemistry and Materials Science
Chitosan
Dairy products
Environmental Engineering/Biotechnology
Enzymatic activity
Enzymes
Epichlorohydrin
Fermentation
Food Science
Fungal Proteins - chemistry
Galactosidase
Glutaraldehyde
Hydrolysis
Immobilization
Industrial and Production Engineering
Industrial Chemistry/Chemical Engineering
Kluyveromyces - enzymology
Kluyveromyces lactis
Lactose
Lactose - chemistry
Lactulose
Lactulose - chemical synthesis
Lactulose - chemistry
Milk
Research Paper
Whey
Whey - chemistry
Yeast
Yeasts
β-Galactosidase
title Simultaneous hydrolysis of cheese whey and lactulose production catalyzed by β-galactosidase from Kluyveromyces lactis NRRL Y1564
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