Rip 1-dependent endothelial necroptosis participates in ischemia-reperfusion injury of mouse flap

•In this study, we show that necroptosis exits in Ischemia reperfusion (I-R) injury of mouse flap.•The necroptosis in I-R injury of mouse flap may be mediated by the membrane receptor TNFR1.•Inhibition of necroptosis substantially improves the survival rate of the flap.•Our data suggest that necroptosis is associated with the development of flap I-R injury and that necroptosis inhibition may contribute to the tolerance of hypoxia, thereby presenting a potential novel therapeutic target for flap I-R injury. Ischemia reperfusion injury plays an important role in free flap necrosis. However, the detailed mechanism is not clear, and effective methods for improving the survival rate of skin flap are still lacking. To investigate the regulation and functional link between necroptosis and ischemia-reperfusion injury of mouse flap. We established a mouse ischemia-reperfusion injury flap model and a cell Oxygen Glucose Deprivation (OGD) model intervened with Necrostatin-1. The mouse flap tissues were harvested in vivo for histological immunofluorescence analysis and western blotting analyses. The HUVECs cells with various treatments in vitro were assessed by using Transwell assay, tube formation assay, cell counting kit-8 analysis and flow cytometry. A Rip3-knockout cell line and a TNFR1-knockout cell line were generated from HUVEC cells using the CRISPR-Cas9 technology and were subsequently used to explore the related mechanisms. The expression of p-Rip3 is positive in both mouse and cell culture models. When necroptosis is completely or partially inhibited in vivo, damaged tissues are repaired with better efficiency. The cells treated with Necrostatin-1 in vitro exhibit faster migration, proliferation and better tube formation. Deficiency of TNFR1 can block the necroptosis pathway by blocking the phosphorylation of Rip3 in HUVEC OGD/ROG model. Meanwhile, the levels of APJ, HIF-1α, and VEGF are reduced when necroptosis is inhibited by Necrostatin-1. TNFR1 mediates Rip1/Rip3 in ischemia-reperfusion injury. Inhibition of necroptosis attenuates the ischemia-reperfusion injury of flap and may enhance hypoxic tolerance of HUVECs and vascular homeostasis through regulation of the HIF-1α signaling pathways.