Pseudomonas carnis sp. nov., isolated from meat

During investigations of spoilage-associated meat microbiota, isolates were found in two different laboratories showing highest similarities to DSM 29167 , DSM 29164 and DSM 18862 based on 16S rRNA gene sequence comparisons. Phylogenetic analysis of the complete gene sequences of isolates B4-1 and SpeckC indicated a separate branch with 99.0 and 99.1 % identity, respectively, to their closest relative ( DSM 29167 ). Further phenotypic and chemotaxonomic characterizations, as well as average nucleotide identity (ANIb) values obtained from the draft genomes, revealed that these isolates could be considered as representing a novel species, with ANIb values of around 94 and 90 % with their closest relatives and . Other related species showed ANIb values below 90 %, including DSM 17149 , DSM 18928 , DSM 17489 , DSM 11331 and DSM 18862 . Genome-to-genome distance calculations between B4-1 and its closest relative, DSM 29167 , showed 62.6 % relatedness. The G+C contents of B4-1 and SpeckC were 59.8 and 59.9 mol%, respectively. The major cellular lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol; the major quinone was Q9. Based on these data, the new species sp. nov. is proposed, the type strain is B4-1 (=DSM 107652 =LMG 30892 ); a second strain is SpeckC (=DSM 107651=LMG 30893).