Continuous microbial production of hydrogen gas

Techniques utilized to study microbial hydrogen gas production involved intermittent flow digesters, chemostats (continuous culture), immobilized cells and immobilized enzymes. Overall, the chemostat appears to have the greatest potential to maximize hydrogen gas productivity. Immobilized systems tended to result in lower hydrogen-producing activities which may have been due to diffusion limitation. Organic compounds, such as carbohydrates, stimulate the greatest amount of H 2.