An aptamer affinity column for purification and enrichment of aflatoxin B1 and aflatoxin B2 in agro-products

We have developed an aptamer affinity column (AAC) for the purification and enrichment of trace aflatoxin B 1 (AFB 1 ) and aflatoxin B 2 (AFB 2 ) in genuine agro-products through the covalent conjugation of amino modified aptamer and NHS-activated Sepharose. The coupling and working conditions found...

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Veröffentlicht in:	Analytical and bioanalytical chemistry 2020-02, Vol.412 (4), p.895-904
Hauptverfasser:	Liu, Hongmei, Zhao, Ying, Lu, Anxiang, Ye, Jin, Wang, Jihua, Wang, Songxue, Luan, Yunxia
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity Aflatoxin B1 Aflatoxins Analytical Chemistry Aptamers Biochemistry Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Conjugation Coupling Food Science High-performance liquid chromatography Laboratory Medicine Linearity Liquid chromatography Monitoring/Environmental Analysis Purification Research Paper Selectivity Working conditions
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Beschreibung
Zusammenfassung:	We have developed an aptamer affinity column (AAC) for the purification and enrichment of trace aflatoxin B 1 (AFB 1 ) and aflatoxin B 2 (AFB 2 ) in genuine agro-products through the covalent conjugation of amino modified aptamer and NHS-activated Sepharose. The coupling and working conditions found to be suitable for this AFB-AAC were examined in regard to coupling time (2 min), loading volume (30 mL), and the methanol concentration (
ISSN:	1618-2642 1618-2650
DOI:	10.1007/s00216-019-02300-4