Seed priming with calcium compounds abrogate fluoride-induced oxidative stress by upregulating defence pathways in an indica rice variety
The aim of this manuscript was to investigate the role of calcium compounds, viz., Ca(OH) 2 , Ca(NO 3 ) 2 , and CaCl 2 (each used at 0.3 mM and 0.5 mM concentration) as seed priming agents to ameliorate fluoride toxicity in rice. The stressed seedlings exhibited high fluoride bioaccumulation, severe...
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| description | The aim of this manuscript was to investigate the role of calcium compounds, viz., Ca(OH)
2
, Ca(NO
3
)
2
, and CaCl
2
(each used at 0.3 mM and 0.5 mM concentration) as seed priming agents to ameliorate fluoride toxicity in rice. The stressed seedlings exhibited high fluoride bioaccumulation, severe growth retardation, and cellular damages. Calcium compounds improved plant performance by increasing seed germination, seedling biomass, and root and shoot length, avoiding chlorophyll degeneration and leakage of electrolytes, along with lowering the levels of malondialdehdye, H
2
O
2
, and endogenous fluoride. Calcium-regulated defence was mediated by proline synthesised due to increased
Δ
1
-pyrroline 5-carboxylate synthetase
(
P5CS
) and lowered
proline dehydrogenase
(
PDH
) expression, and glycine betaine synthesised due to
betaine aldehyde dehydrogenase 1
(
BADH1
) expression. While the stress-mediated lowering of carotenoids and total phenolics was relieved by calcium priming, stress-enhanced flavonoids and ascorbic acid content was restored to the normal condition, along with releasing the fluoride-induced inhibition of ascorbic acid oxidase (AAO) activity. The activities of antioxidant enzymes like catalase, guaiacol peroxidase, and superoxide dismutase, and the expression of
catalase
and
superoxide dismutase
genes were also affected by calcium priming. The elevated endogenous calcium level, brought about by priming, enhanced the expression of genes related to calcium signalling pathway, particularly the
calcineurin-B-like 10
(
CBL10
) gene. Ca(OH)
2
(0.3 mM) appeared to be the most efficient of all the three priming agents. Overall, the present work highlighted the efficacy of calcium compounds as priming agents in abrogating fluoride toxicity in rice. |
| doi_str_mv | 10.1007/s00709-019-01460-5 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2328755741</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2399055176</sourcerecordid><originalsourceid>FETCH-LOGICAL-c375t-7498d6e29880b14c37aee51742fbdd4c717d1fd7d5abe900adfb8a3a997dc48e3</originalsourceid><addsrcrecordid>eNp9kc1u1TAQha0KRC8tL9AFssSGTWAcx3GyrCr-pEosAKk7y7Ent64SO7XjlvsIvDW-3EKlLlh4LM1854zlQ8gZg3cMQL5PpUBfAdufpoVKHJENa5moWgb1M7IB4LxiHb86Ji9TugEAUYN4QY456wRvm3ZDfn1DtHSJbnZ-S-_dek2NnozLMzVhXkL2NlE9xLDVK9JxyiE6i5XzNpsiDD-d1au7Q5rWiCnRYUfzEnGbp9IujhZH9Abpotfre71L1HmqfanWGU2jK6M7HR2uu1PyfNRTwlcP9wn58fHD94vP1eXXT18uzi8rw6VYK9n0nW2x7rsOBtaUpkYUTDb1OFjbGMmkZaOVVugBewBtx6HTXPe9tKbpkJ-QtwffJYbbjGlVs0sGp0l7DDmpmtedFEI2rKBvnqA3IUdfXleovgdR9raFqg-UiSGliKPaf6eOO8VA7YNSh6BUCUr9CUqJInr9YJ2HGe0_yd9kCsAPQCojv8X4uPs_tr8BoNShTw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2399055176</pqid></control><display><type>article</type><title>Seed priming with calcium compounds abrogate fluoride-induced oxidative stress by upregulating defence pathways in an indica rice variety</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Singh, Ankur ; Banerjee, Aditya ; Roychoudhury, Aryadeep</creator><creatorcontrib>Singh, Ankur ; Banerjee, Aditya ; Roychoudhury, Aryadeep</creatorcontrib><description>The aim of this manuscript was to investigate the role of calcium compounds, viz., Ca(OH)
2
, Ca(NO
3
)
2
, and CaCl
2
(each used at 0.3 mM and 0.5 mM concentration) as seed priming agents to ameliorate fluoride toxicity in rice. The stressed seedlings exhibited high fluoride bioaccumulation, severe growth retardation, and cellular damages. Calcium compounds improved plant performance by increasing seed germination, seedling biomass, and root and shoot length, avoiding chlorophyll degeneration and leakage of electrolytes, along with lowering the levels of malondialdehdye, H
2
O
2
, and endogenous fluoride. Calcium-regulated defence was mediated by proline synthesised due to increased
Δ
1
-pyrroline 5-carboxylate synthetase
(
P5CS
) and lowered
proline dehydrogenase
(
PDH
) expression, and glycine betaine synthesised due to
betaine aldehyde dehydrogenase 1
(
BADH1
) expression. While the stress-mediated lowering of carotenoids and total phenolics was relieved by calcium priming, stress-enhanced flavonoids and ascorbic acid content was restored to the normal condition, along with releasing the fluoride-induced inhibition of ascorbic acid oxidase (AAO) activity. The activities of antioxidant enzymes like catalase, guaiacol peroxidase, and superoxide dismutase, and the expression of
catalase
and
superoxide dismutase
genes were also affected by calcium priming. The elevated endogenous calcium level, brought about by priming, enhanced the expression of genes related to calcium signalling pathway, particularly the
calcineurin-B-like 10
(
CBL10
) gene. Ca(OH)
2
(0.3 mM) appeared to be the most efficient of all the three priming agents. Overall, the present work highlighted the efficacy of calcium compounds as priming agents in abrogating fluoride toxicity in rice.</description><identifier>ISSN: 0033-183X</identifier><identifier>EISSN: 1615-6102</identifier><identifier>DOI: 10.1007/s00709-019-01460-5</identifier><identifier>PMID: 31853646</identifier><language>eng</language><publisher>Vienna: Springer Vienna</publisher><subject>Aldehyde dehydrogenase ; Antioxidants ; Ascorbic acid ; Bioaccumulation ; Biomedical and Life Sciences ; Calcineurin ; Calcium - chemistry ; Calcium chloride ; Calcium hydroxide ; Calcium signalling ; Carotenoids ; Catalase ; Cell Biology ; Chlorophyll ; Degeneration ; Dehydrogenases ; Flavonoids ; Fluorides ; Glycine betaine ; Growth rate ; Guaiacol ; Hydrogen peroxide ; Life Sciences ; Original Article ; Oryza - chemistry ; Oxidative Stress ; Peroxidase ; Plant Sciences ; Proline ; Seeds - chemistry ; Signal transduction ; Superoxide dismutase ; Toxicity ; Up-Regulation ; Zoology</subject><ispartof>Protoplasma, 2020-05, Vol.257 (3), p.767-782</ispartof><rights>Springer-Verlag GmbH Austria, part of Springer Nature 2019</rights><rights>Springer-Verlag GmbH Austria, part of Springer Nature 2019.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-7498d6e29880b14c37aee51742fbdd4c717d1fd7d5abe900adfb8a3a997dc48e3</citedby><cites>FETCH-LOGICAL-c375t-7498d6e29880b14c37aee51742fbdd4c717d1fd7d5abe900adfb8a3a997dc48e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00709-019-01460-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00709-019-01460-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31853646$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Singh, Ankur</creatorcontrib><creatorcontrib>Banerjee, Aditya</creatorcontrib><creatorcontrib>Roychoudhury, Aryadeep</creatorcontrib><title>Seed priming with calcium compounds abrogate fluoride-induced oxidative stress by upregulating defence pathways in an indica rice variety</title><title>Protoplasma</title><addtitle>Protoplasma</addtitle><addtitle>Protoplasma</addtitle><description>The aim of this manuscript was to investigate the role of calcium compounds, viz., Ca(OH)
2
, Ca(NO
3
)
2
, and CaCl
2
(each used at 0.3 mM and 0.5 mM concentration) as seed priming agents to ameliorate fluoride toxicity in rice. The stressed seedlings exhibited high fluoride bioaccumulation, severe growth retardation, and cellular damages. Calcium compounds improved plant performance by increasing seed germination, seedling biomass, and root and shoot length, avoiding chlorophyll degeneration and leakage of electrolytes, along with lowering the levels of malondialdehdye, H
2
O
2
, and endogenous fluoride. Calcium-regulated defence was mediated by proline synthesised due to increased
Δ
1
-pyrroline 5-carboxylate synthetase
(
P5CS
) and lowered
proline dehydrogenase
(
PDH
) expression, and glycine betaine synthesised due to
betaine aldehyde dehydrogenase 1
(
BADH1
) expression. While the stress-mediated lowering of carotenoids and total phenolics was relieved by calcium priming, stress-enhanced flavonoids and ascorbic acid content was restored to the normal condition, along with releasing the fluoride-induced inhibition of ascorbic acid oxidase (AAO) activity. The activities of antioxidant enzymes like catalase, guaiacol peroxidase, and superoxide dismutase, and the expression of
catalase
and
superoxide dismutase
genes were also affected by calcium priming. The elevated endogenous calcium level, brought about by priming, enhanced the expression of genes related to calcium signalling pathway, particularly the
calcineurin-B-like 10
(
CBL10
) gene. Ca(OH)
2
(0.3 mM) appeared to be the most efficient of all the three priming agents. Overall, the present work highlighted the efficacy of calcium compounds as priming agents in abrogating fluoride toxicity in rice.</description><subject>Aldehyde dehydrogenase</subject><subject>Antioxidants</subject><subject>Ascorbic acid</subject><subject>Bioaccumulation</subject><subject>Biomedical and Life Sciences</subject><subject>Calcineurin</subject><subject>Calcium - chemistry</subject><subject>Calcium chloride</subject><subject>Calcium hydroxide</subject><subject>Calcium signalling</subject><subject>Carotenoids</subject><subject>Catalase</subject><subject>Cell Biology</subject><subject>Chlorophyll</subject><subject>Degeneration</subject><subject>Dehydrogenases</subject><subject>Flavonoids</subject><subject>Fluorides</subject><subject>Glycine betaine</subject><subject>Growth rate</subject><subject>Guaiacol</subject><subject>Hydrogen peroxide</subject><subject>Life Sciences</subject><subject>Original Article</subject><subject>Oryza - chemistry</subject><subject>Oxidative Stress</subject><subject>Peroxidase</subject><subject>Plant Sciences</subject><subject>Proline</subject><subject>Seeds - chemistry</subject><subject>Signal transduction</subject><subject>Superoxide dismutase</subject><subject>Toxicity</subject><subject>Up-Regulation</subject><subject>Zoology</subject><issn>0033-183X</issn><issn>1615-6102</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kc1u1TAQha0KRC8tL9AFssSGTWAcx3GyrCr-pEosAKk7y7Ent64SO7XjlvsIvDW-3EKlLlh4LM1854zlQ8gZg3cMQL5PpUBfAdufpoVKHJENa5moWgb1M7IB4LxiHb86Ji9TugEAUYN4QY456wRvm3ZDfn1DtHSJbnZ-S-_dek2NnozLMzVhXkL2NlE9xLDVK9JxyiE6i5XzNpsiDD-d1au7Q5rWiCnRYUfzEnGbp9IujhZH9Abpotfre71L1HmqfanWGU2jK6M7HR2uu1PyfNRTwlcP9wn58fHD94vP1eXXT18uzi8rw6VYK9n0nW2x7rsOBtaUpkYUTDb1OFjbGMmkZaOVVugBewBtx6HTXPe9tKbpkJ-QtwffJYbbjGlVs0sGp0l7DDmpmtedFEI2rKBvnqA3IUdfXleovgdR9raFqg-UiSGliKPaf6eOO8VA7YNSh6BUCUr9CUqJInr9YJ2HGe0_yd9kCsAPQCojv8X4uPs_tr8BoNShTw</recordid><startdate>20200501</startdate><enddate>20200501</enddate><creator>Singh, Ankur</creator><creator>Banerjee, Aditya</creator><creator>Roychoudhury, Aryadeep</creator><general>Springer Vienna</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88G</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2M</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20200501</creationdate><title>Seed priming with calcium compounds abrogate fluoride-induced oxidative stress by upregulating defence pathways in an indica rice variety</title><author>Singh, Ankur ; Banerjee, Aditya ; Roychoudhury, Aryadeep</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-7498d6e29880b14c37aee51742fbdd4c717d1fd7d5abe900adfb8a3a997dc48e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Aldehyde dehydrogenase</topic><topic>Antioxidants</topic><topic>Ascorbic acid</topic><topic>Bioaccumulation</topic><topic>Biomedical and Life Sciences</topic><topic>Calcineurin</topic><topic>Calcium - chemistry</topic><topic>Calcium chloride</topic><topic>Calcium hydroxide</topic><topic>Calcium signalling</topic><topic>Carotenoids</topic><topic>Catalase</topic><topic>Cell Biology</topic><topic>Chlorophyll</topic><topic>Degeneration</topic><topic>Dehydrogenases</topic><topic>Flavonoids</topic><topic>Fluorides</topic><topic>Glycine betaine</topic><topic>Growth rate</topic><topic>Guaiacol</topic><topic>Hydrogen peroxide</topic><topic>Life Sciences</topic><topic>Original Article</topic><topic>Oryza - chemistry</topic><topic>Oxidative Stress</topic><topic>Peroxidase</topic><topic>Plant Sciences</topic><topic>Proline</topic><topic>Seeds - chemistry</topic><topic>Signal transduction</topic><topic>Superoxide dismutase</topic><topic>Toxicity</topic><topic>Up-Regulation</topic><topic>Zoology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Singh, Ankur</creatorcontrib><creatorcontrib>Banerjee, Aditya</creatorcontrib><creatorcontrib>Roychoudhury, Aryadeep</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Psychology Database (Alumni)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Psychology</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest One Psychology</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Protoplasma</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Singh, Ankur</au><au>Banerjee, Aditya</au><au>Roychoudhury, Aryadeep</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Seed priming with calcium compounds abrogate fluoride-induced oxidative stress by upregulating defence pathways in an indica rice variety</atitle><jtitle>Protoplasma</jtitle><stitle>Protoplasma</stitle><addtitle>Protoplasma</addtitle><date>2020-05-01</date><risdate>2020</risdate><volume>257</volume><issue>3</issue><spage>767</spage><epage>782</epage><pages>767-782</pages><issn>0033-183X</issn><eissn>1615-6102</eissn><abstract>The aim of this manuscript was to investigate the role of calcium compounds, viz., Ca(OH)
2
, Ca(NO
3
)
2
, and CaCl
2
(each used at 0.3 mM and 0.5 mM concentration) as seed priming agents to ameliorate fluoride toxicity in rice. The stressed seedlings exhibited high fluoride bioaccumulation, severe growth retardation, and cellular damages. Calcium compounds improved plant performance by increasing seed germination, seedling biomass, and root and shoot length, avoiding chlorophyll degeneration and leakage of electrolytes, along with lowering the levels of malondialdehdye, H
2
O
2
, and endogenous fluoride. Calcium-regulated defence was mediated by proline synthesised due to increased
Δ
1
-pyrroline 5-carboxylate synthetase
(
P5CS
) and lowered
proline dehydrogenase
(
PDH
) expression, and glycine betaine synthesised due to
betaine aldehyde dehydrogenase 1
(
BADH1
) expression. While the stress-mediated lowering of carotenoids and total phenolics was relieved by calcium priming, stress-enhanced flavonoids and ascorbic acid content was restored to the normal condition, along with releasing the fluoride-induced inhibition of ascorbic acid oxidase (AAO) activity. The activities of antioxidant enzymes like catalase, guaiacol peroxidase, and superoxide dismutase, and the expression of
catalase
and
superoxide dismutase
genes were also affected by calcium priming. The elevated endogenous calcium level, brought about by priming, enhanced the expression of genes related to calcium signalling pathway, particularly the
calcineurin-B-like 10
(
CBL10
) gene. Ca(OH)
2
(0.3 mM) appeared to be the most efficient of all the three priming agents. Overall, the present work highlighted the efficacy of calcium compounds as priming agents in abrogating fluoride toxicity in rice.</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><pmid>31853646</pmid><doi>10.1007/s00709-019-01460-5</doi><tpages>16</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0033-183X |
| ispartof | Protoplasma, 2020-05, Vol.257 (3), p.767-782 |
| issn | 0033-183X 1615-6102 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2328755741 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Aldehyde dehydrogenase Antioxidants Ascorbic acid Bioaccumulation Biomedical and Life Sciences Calcineurin Calcium - chemistry Calcium chloride Calcium hydroxide Calcium signalling Carotenoids Catalase Cell Biology Chlorophyll Degeneration Dehydrogenases Flavonoids Fluorides Glycine betaine Growth rate Guaiacol Hydrogen peroxide Life Sciences Original Article Oryza - chemistry Oxidative Stress Peroxidase Plant Sciences Proline Seeds - chemistry Signal transduction Superoxide dismutase Toxicity Up-Regulation Zoology |
| title | Seed priming with calcium compounds abrogate fluoride-induced oxidative stress by upregulating defence pathways in an indica rice variety |
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