Lacibacter luteus sp. nov., isolated from a freshwater river

An isolate, designated TTM-7 , recovered from a freshwater river in Taiwan, was characterized using a polyphasic taxonomy approach. Phylogenetic analyses based on 16S rRNA gene sequences and an up-to-date bacterial core gene set (92 protein clusters) indicated that strain TTM-7 is affiliated with species in the genus . Strain TTM-7 was most closely related to NJ-8 with a 98.2 % 16S rRNA gene sequence similarity, followed by H32-4 (97.4 %) and SS2-56 (96.3 %). Cells were Gram-stain-negative, aerobic, motile by gliding, rod-shaped and formed orange colonies. Optimal growth occurred at 25 °C, pH 7 and 0 % NaCl. The major fatty acids of strain TTM-7 were iso-C , summed feature 3 (comprising C ω c/C ω and/or iso-C 2-OH) and iso-C G. The predominant hydroxy fatty acid was iso-C 3-OH. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, two unidentified aminoglycolipids, five unidentified aminophospholipids and one unidentified lipid. The major isoprenoid quinone was MK-7. Genomic DNA G+C content of strain TTM-7 was 40.6 mol%. Strain TTM-7 showed 83.6 % average nucleotide identity and 16.0 % digital DNA-DNA hybridization identity with NJ-8 . On the basis of phenotypic and genotypic properties and phylogenetic inference, strain TTM-7 should be classified in a novel species of the genus , for which the name sp. nov. is proposed. The type strain is TTM-7 (=BCRC 81159 =LMG 30926 =KCTC 62870 ).