Integrated Microstructured Photonic Fiber as a Bifunctional Robust Frit and Efficient Electrospray Emitter of a Packed Column for Capillary Liquid Chromatography–Tandem Mass Spectrometry Analysis of Complex Biological Samples
Although capillary liquid chromatography married with tandem mass spectrometry (cLC–MS/MS) has become a powerful technique for proteomics and metabolomics research, it is still a great challenge to fabricate durable capillary-based analytical columns coupling continuous nanoflow (100), which always...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2020-01, Vol.92 (2), p.2274-2282 |
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| creator | Ma, Shujuan Wang, Yan Zhang, Na Lyu, Jiawen Ma, Chen Xu, Junwen Li, Xiaowei Ou, Junjie Ye, Mingliang |
| description | Although capillary liquid chromatography married with tandem mass spectrometry (cLC–MS/MS) has become a powerful technique for proteomics and metabolomics research, it is still a great challenge to fabricate durable capillary-based analytical columns coupling continuous nanoflow (100), which always encumbers both independent and integral tapered tip emitters for cLC–MS. |
| doi_str_mv | 10.1021/acs.analchem.9b04997 |
| format | Article |
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Here, we proposed a simple approach to integrate microstructured photonic fibers (MPFs) into wide bore capillaries with 150 μm i.d., serving as an integral bifunctional frit or/and ESI emitter of packed columns. Two kinds of MPFs containing 126 homogeneous microchannels with different inner diameter, 3.2 μm for MPF-1 and 2.6 μm for MPF-2, were explored for preparation. The octadecylsilicate (ODS) silica-packed column using MPF-1 as a frit exhibited the lowest plate heights of 14.2–19.7 μm for five alkylbenzenes at the velocity of 1.5 mm/s, which were slightly lower than those of packed column with porous polymer monolith (PPM)-based frit by cLC coupling with ultraviolet (UV) detection. Additionally, the packed columns with integral MPF frit-emitters were further applied in analysis of a complex biological sample of digest of Hela cells by cLC–MS. An average of 7109 unique peptides could be identified in a single analysis by using MPF-1 emitter, and 7110 unique peptides were identified by using the MPF-2 emitter, which were superior to the identified result of packed column with an integral tapered tip emitter (6894 peptides). It is obvious that this novel integral MPF-based frit-emitter does not easily suffer from the issues of cracking owing to the silica cladding around independent microchannels (>100), which always encumbers both independent and integral tapered tip emitters for cLC–MS.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/acs.analchem.9b04997</identifier><identifier>PMID: 31846285</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Alkylbenzenes ; Biological properties ; Biological samples ; Boring ; Capillaries ; Chemistry ; Chromatography ; Chromatography, Liquid ; Column chromatography ; Coupling (molecular) ; Electrospraying ; Emitters ; Fibers ; Frit ; HeLa Cells ; Humans ; Integrals ; Ionization ; Ions ; Liquid chromatography ; Mass spectrometry ; Mass spectroscopy ; Metabolomics ; Microchannels ; Optical Fibers ; Packed columns ; Particle Size ; Peptides ; Peptides - analysis ; Photonics ; Photons ; Polymers ; Proteins - analysis ; Proteomics ; Scientific imaging ; Silica ; Silicon dioxide ; Spectrometry, Mass, Electrospray Ionization ; Spectroscopy ; Surface Properties ; Tandem Mass Spectrometry ; Tumor Cells, Cultured ; Ultraviolet Rays</subject><ispartof>Analytical chemistry (Washington), 2020-01, Vol.92 (2), p.2274-2282</ispartof><rights>Copyright American Chemical Society Jan 21, 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a413t-40af3bedb198027e14c00b9709949c4e2fbef39357d82c1bcba33905b34e0c1f3</citedby><cites>FETCH-LOGICAL-a413t-40af3bedb198027e14c00b9709949c4e2fbef39357d82c1bcba33905b34e0c1f3</cites><orcidid>0000-0002-5872-9326 ; 0000-0002-1672-6119</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.analchem.9b04997$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.analchem.9b04997$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31846285$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ma, Shujuan</creatorcontrib><creatorcontrib>Wang, Yan</creatorcontrib><creatorcontrib>Zhang, Na</creatorcontrib><creatorcontrib>Lyu, Jiawen</creatorcontrib><creatorcontrib>Ma, Chen</creatorcontrib><creatorcontrib>Xu, Junwen</creatorcontrib><creatorcontrib>Li, Xiaowei</creatorcontrib><creatorcontrib>Ou, Junjie</creatorcontrib><creatorcontrib>Ye, Mingliang</creatorcontrib><title>Integrated Microstructured Photonic Fiber as a Bifunctional Robust Frit and Efficient Electrospray Emitter of a Packed Column for Capillary Liquid Chromatography–Tandem Mass Spectrometry Analysis of Complex Biological Samples</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>Although capillary liquid chromatography married with tandem mass spectrometry (cLC–MS/MS) has become a powerful technique for proteomics and metabolomics research, it is still a great challenge to fabricate durable capillary-based analytical columns coupling continuous nanoflow (<1 000 nL/min) electrospray ionization (ESI) with MS, owing to the issue of clogging and fragile of emitters. Here, we proposed a simple approach to integrate microstructured photonic fibers (MPFs) into wide bore capillaries with 150 μm i.d., serving as an integral bifunctional frit or/and ESI emitter of packed columns. Two kinds of MPFs containing 126 homogeneous microchannels with different inner diameter, 3.2 μm for MPF-1 and 2.6 μm for MPF-2, were explored for preparation. The octadecylsilicate (ODS) silica-packed column using MPF-1 as a frit exhibited the lowest plate heights of 14.2–19.7 μm for five alkylbenzenes at the velocity of 1.5 mm/s, which were slightly lower than those of packed column with porous polymer monolith (PPM)-based frit by cLC coupling with ultraviolet (UV) detection. Additionally, the packed columns with integral MPF frit-emitters were further applied in analysis of a complex biological sample of digest of Hela cells by cLC–MS. An average of 7109 unique peptides could be identified in a single analysis by using MPF-1 emitter, and 7110 unique peptides were identified by using the MPF-2 emitter, which were superior to the identified result of packed column with an integral tapered tip emitter (6894 peptides). It is obvious that this novel integral MPF-based frit-emitter does not easily suffer from the issues of cracking owing to the silica cladding around independent microchannels (>100), which always encumbers both independent and integral tapered tip emitters for cLC–MS.</description><subject>Alkylbenzenes</subject><subject>Biological properties</subject><subject>Biological samples</subject><subject>Boring</subject><subject>Capillaries</subject><subject>Chemistry</subject><subject>Chromatography</subject><subject>Chromatography, Liquid</subject><subject>Column chromatography</subject><subject>Coupling (molecular)</subject><subject>Electrospraying</subject><subject>Emitters</subject><subject>Fibers</subject><subject>Frit</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Integrals</subject><subject>Ionization</subject><subject>Ions</subject><subject>Liquid chromatography</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Metabolomics</subject><subject>Microchannels</subject><subject>Optical Fibers</subject><subject>Packed columns</subject><subject>Particle Size</subject><subject>Peptides</subject><subject>Peptides - analysis</subject><subject>Photonics</subject><subject>Photons</subject><subject>Polymers</subject><subject>Proteins - analysis</subject><subject>Proteomics</subject><subject>Scientific imaging</subject><subject>Silica</subject><subject>Silicon dioxide</subject><subject>Spectrometry, Mass, Electrospray Ionization</subject><subject>Spectroscopy</subject><subject>Surface Properties</subject><subject>Tandem Mass Spectrometry</subject><subject>Tumor Cells, Cultured</subject><subject>Ultraviolet Rays</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9ks1uEzEUhUcIREvhDRCyxIZNwvXPZMbLEiVQKRUVLeuR7diNy8x4ansksuMdeEN4Ee6QtAsWrCzb53z3XPsWxWsKcwqMvlcmzVWvWrOz3VxqEFJWT4pTWjKYLeqaPS1OAYDPWAVwUrxI6Q6AUqCL58UJp7VYsLo8LX5f9NneRpXtllx6E0PKcTR5jLi_2oUcem_I2msbiUpEkQ_ejb3JPmBl8iXoMWWyjj4T1W_JyjlvvO0zWbXWZIQNUe3JqvM5IyA4BFwp8w3Zy9COXU9ciGSpBt-2Ku7Jxt-PHu92MXQqB4w17Pa_fvy8QbjtyKVKiVwPf8mdzWg4xxT75NOEXoZuaO13TBjacOsN5rtW01F6WTxzqk321XE9K76uVzfLT7PN548Xy_PNTAnK80yAclzbraayBlZZKgyAlhVIKaQRljltHZe8rLY1M1QbrTiXUGouLBjq-Fnx7sAdYrgfbcpN55Ox2Ftvw5gaxlnNRSkrjtK3_0jvwhixm0kl-KKSrCxRJQ6q6V9StK4Zou_wpRoKzTQEDQ5B8zAEzXEI0PbmCB91Z7ePpodfRwEcBJP9sfB_mX8A1OfHgQ</recordid><startdate>20200121</startdate><enddate>20200121</enddate><creator>Ma, Shujuan</creator><creator>Wang, Yan</creator><creator>Zhang, Na</creator><creator>Lyu, Jiawen</creator><creator>Ma, Chen</creator><creator>Xu, Junwen</creator><creator>Li, Xiaowei</creator><creator>Ou, Junjie</creator><creator>Ye, Mingliang</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-5872-9326</orcidid><orcidid>https://orcid.org/0000-0002-1672-6119</orcidid></search><sort><creationdate>20200121</creationdate><title>Integrated Microstructured Photonic Fiber as a Bifunctional Robust Frit and Efficient Electrospray Emitter of a Packed Column for Capillary Liquid Chromatography–Tandem Mass Spectrometry Analysis of Complex Biological Samples</title><author>Ma, Shujuan ; 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Chem</addtitle><date>2020-01-21</date><risdate>2020</risdate><volume>92</volume><issue>2</issue><spage>2274</spage><epage>2282</epage><pages>2274-2282</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><abstract>Although capillary liquid chromatography married with tandem mass spectrometry (cLC–MS/MS) has become a powerful technique for proteomics and metabolomics research, it is still a great challenge to fabricate durable capillary-based analytical columns coupling continuous nanoflow (<1 000 nL/min) electrospray ionization (ESI) with MS, owing to the issue of clogging and fragile of emitters. Here, we proposed a simple approach to integrate microstructured photonic fibers (MPFs) into wide bore capillaries with 150 μm i.d., serving as an integral bifunctional frit or/and ESI emitter of packed columns. Two kinds of MPFs containing 126 homogeneous microchannels with different inner diameter, 3.2 μm for MPF-1 and 2.6 μm for MPF-2, were explored for preparation. The octadecylsilicate (ODS) silica-packed column using MPF-1 as a frit exhibited the lowest plate heights of 14.2–19.7 μm for five alkylbenzenes at the velocity of 1.5 mm/s, which were slightly lower than those of packed column with porous polymer monolith (PPM)-based frit by cLC coupling with ultraviolet (UV) detection. Additionally, the packed columns with integral MPF frit-emitters were further applied in analysis of a complex biological sample of digest of Hela cells by cLC–MS. An average of 7109 unique peptides could be identified in a single analysis by using MPF-1 emitter, and 7110 unique peptides were identified by using the MPF-2 emitter, which were superior to the identified result of packed column with an integral tapered tip emitter (6894 peptides). It is obvious that this novel integral MPF-based frit-emitter does not easily suffer from the issues of cracking owing to the silica cladding around independent microchannels (>100), which always encumbers both independent and integral tapered tip emitters for cLC–MS.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>31846285</pmid><doi>10.1021/acs.analchem.9b04997</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-5872-9326</orcidid><orcidid>https://orcid.org/0000-0002-1672-6119</orcidid></addata></record> |
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| subjects | Alkylbenzenes Biological properties Biological samples Boring Capillaries Chemistry Chromatography Chromatography, Liquid Column chromatography Coupling (molecular) Electrospraying Emitters Fibers Frit HeLa Cells Humans Integrals Ionization Ions Liquid chromatography Mass spectrometry Mass spectroscopy Metabolomics Microchannels Optical Fibers Packed columns Particle Size Peptides Peptides - analysis Photonics Photons Polymers Proteins - analysis Proteomics Scientific imaging Silica Silicon dioxide Spectrometry, Mass, Electrospray Ionization Spectroscopy Surface Properties Tandem Mass Spectrometry Tumor Cells, Cultured Ultraviolet Rays |
| title | Integrated Microstructured Photonic Fiber as a Bifunctional Robust Frit and Efficient Electrospray Emitter of a Packed Column for Capillary Liquid Chromatography–Tandem Mass Spectrometry Analysis of Complex Biological Samples |
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