Complete genome sequence of a novel victorivirus isolated from the sesame charcoal rot fungus Macrophomina phaseolina

Macrophomina phaseolina is an important phytopathogenic fungus with a broad host range. Here, the complete genome sequence of a novel victorivirus, tentatively named M acrophomina p haseolina v ictorivirus 1 (MpV1), was identified from strain 2012-019 of M. phaseolina . The MpV1 genome is 5,128 nucleotides long with a predicted GC content of 62%. Sequence analysis indicated that two open reading frames (ORF 1 and 2) overlap at a tetranucleotide AUGA sequence. Proteins encoded by ORF1 and ORF2 showed significant sequence similarity to coat proteins and the RNA-dependent RNA polymerases, respectively, of members of the family Totiviridae . Analysis of the genomic structure of MpV1, homolog searches of the deduced amino acid sequences, and phylogenetic analysis indicated that MpV1 is a new member of the genus Victorivirus . As far as we know, this is the first report of the full-length nucleotide sequence of the genome of a novel victorivirus that infects M. phaseolina .