Chryseotalea sanaruensis gen. nov., sp., nov., a Member of the Family Cytophagaceae, Isolated from a Brackish Lake in Hamamatsu Japan

A strain-designated Ys T was isolated as a filterable bacterial strain from Lake Sanaru, a brackish water lake in Hamamatsu Japan. Ys T is aerobic, Gram-negative, and slender rod shaped. Ys T grew optimally at 30 °C, pH 7.0–8.0 and without the addition of NaCl. MK-7 was the sole isoprenoid quinone. The main cellular polar lipids were phosphatidylethanolamine and unidentified amino- and polar-lipids. The predominant cellular fatty acids were C 18:0 , iso -C 14:0 and iso -C 15:0 . Phylogenetic analysis of 16S rRNA gene sequence revealed the nearest neighbours of strain Ys T to be members of the Ohtaekwangia and Chryseolinea genera with 91.2–92.1% sequence similarity. The percentages of conserved proteins (POCP) between the genomes of Ys T and related strains were less than 50%. Phenotypic analyses suggested that Ys T could not metabolize glucose and related sugars, which was discriminative from its phylogenetic relatives. We, therefore, propose a novel species in a new genus, Chryseotalea sanaruensis gen. nov., sp. nov. in the family Cytophagaceae (= JCM 30318 T = LMG 30359 T ), based on cell size, the predominant cellular fatty acid composition, and the DNA GC content (38.9 mol%).