Effect of Blocking the OX40/OX40L Signaling Pathway by siRNA Interference on Animal Experimental Study of Allergic Rhinitis

The identification of new approaches and intervention targets for the treatment of AR is urgently needed. We aimed to investigate the effect of blocking the OX40/OX40L signaling pathway by small interfering RNA (siRNA) on ovalbumin (OVA)-induced AR in a mouse model. After establishment of the AR mod...

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Veröffentlicht in:	Archives of medical research 2019-10, Vol.50 (7), p.442-450
Hauptverfasser:	Liang, Zhuo-ping, Xu, Wei, Zhao, Fei-peng, Pan, Hong-zhu, Feng, Hua-jun, Xu, Sheng-en, Zhao, Chong, Bao, Yi-lin, Jiang, Liang, Huang, Ying, Qin, Gang
Format:	Artikel
Sprache:	eng
Schlagworte:	Allergic rhinitis Animals Dendritic cell Disease Models, Animal Mechanism Mice Ovalbumin - genetics OX40 Ligand Rhinitis, Allergic - genetics RNA, Small Interfering - genetics Signal Transduction Small interfering RNA
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Beschreibung
Zusammenfassung:	The identification of new approaches and intervention targets for the treatment of AR is urgently needed. We aimed to investigate the effect of blocking the OX40/OX40L signaling pathway by small interfering RNA (siRNA) on ovalbumin (OVA)-induced AR in a mouse model. After establishment of the AR model, the mice were interfered by siRNA-OX40L (experimental group), siRNA-C (negative control group), or PBS (control group). Nose scratching, sneezing and nasal discharge were observed. OX40L mRNA and protein and the IL-5, TNF-α, regulatory T cell (Treg) -specific marker Foxp3, and eosinophil (EOS) levels were analyzed. The numbers of nose scratching and sneezing were significantly lower in the siRNA-OX40L-treated group (p
ISSN:	0188-4409 1873-5487
DOI:	10.1016/j.arcmed.2019.11.003